Comparison of Dot-Elisa with Sandwich-Elisa for the Detection of Circulating Antigens in Patients with Bancroftian Filariasis

Zheng Hui-Jun Guizhou Provincial Institute of Parasitic Diseases, Harvard School of Public Health, Guiyang, People's Republic of China

Search for other papers by Zheng Hui-Jun in
Current site
Google Scholar
PubMed Close
,
Tao Zheng-Hou Guizhou Provincial Institute of Parasitic Diseases, Harvard School of Public Health, Guiyang, People's Republic of China

Search for other papers by Tao Zheng-Hou in
Current site
Google Scholar
PubMed Close
,
Cheng Weng-Feng Guizhou Provincial Institute of Parasitic Diseases, Harvard School of Public Health, Guiyang, People's Republic of China

Search for other papers by Cheng Weng-Feng in
Current site
Google Scholar
PubMed Close
, and
Willy F. Piessens Guizhou Provincial Institute of Parasitic Diseases, Harvard School of Public Health, Guiyang, People's Republic of China

Search for other papers by Willy F. Piessens in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1990.42.546
Volume/Issue:
Volume 42: Issue 6
Page(s):
546–549
Restricted access
Get Permissions

We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80–1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10–1:320. The specificity of both assays was >95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.

Author Notes

Copyright:
Copyright © 1990 by The American Society of Tropical Medicine and Hygiene 1990
Published Online:
Jun 1990
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 1583 1040 58
Full Text Views 10 0 0
PDF Downloads 11 0 0
 

 

 

 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save