Protection of Mice Against Lethal Japanese Encephalitis with a Recombinant Baculovirus Vaccine

Jack McCown Walter Reed Army Institute of Research, MicroGeneSys Inc., Washington, DC

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Mark Cochran Walter Reed Army Institute of Research, MicroGeneSys Inc., Washington, DC

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Robert Putnak Walter Reed Army Institute of Research, MicroGeneSys Inc., Washington, DC

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Robert Feighny Walter Reed Army Institute of Research, MicroGeneSys Inc., Washington, DC

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Jeanne Burrous Walter Reed Army Institute of Research, MicroGeneSys Inc., Washington, DC

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Erik Henchal Walter Reed Army Institute of Research, MicroGeneSys Inc., Washington, DC

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Charles Hoke Walter Reed Army Institute of Research, MicroGeneSys Inc., Washington, DC

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DOI:
https://doi.org/10.4269/ajtmh.1990.42.491
Volume/Issue:
Volume 42: Issue 5
Page(s):
491–499
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Genes coding for the E and NS1 glycoproteins of Japanese encephalitis virus (JEV) were cloned into baculovirus expression vectors. The recombinant baculoviruses obtained were used to infect Spodoptera frugiperda cells. The infected cells were used to immunize C57/B mice, which were then challenged with live JEV. Survival was increased from about 30% in unimmunized mice to 70% in E and polyprotein recipients (P < 0.005), but was not increased in NS1 recipients despite the development of antibody against NS1 by these mice. Virus neutralizing antibody was demonstrated in 18/20 E glycoprotein recipients and 15/20 polyprotein recipients. The baculovirus expressed E glycoprotein stimulated antibody which was protective and neutralizing in this system.
Copyright:
Copyright © 1990 by The American Society of Tropical Medicine and Hygiene 1990
Published Online:
May 1990
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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