| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 876 | 555 | 24 |
| Full Text Views | 54 | 21 | 0 |
| PDF Downloads | 47 | 14 | 0 |
Identification of Monoclonal Antibodies Capable of Differentiating Antigenic Varieties of Eastern Equine Encephalitis Viruses
J. T. Roehrig
J. T. Roehrig
Center for Infectious Diseases, Centers for Disease Control, National Institute of Virology, Fort Collins, Colorado, India
Search for other papers by J. T. Roehrig in
Current site
Google Scholar
PubMed
Search for other papers by J. T. Roehrig in
Current site
Google Scholar
PubMed
A. R. Hunt
A. R. Hunt
Center for Infectious Diseases, Centers for Disease Control, National Institute of Virology, Fort Collins, Colorado, India
Search for other papers by A. R. Hunt in
Current site
Google Scholar
PubMed
Search for other papers by A. R. Hunt in
Current site
Google Scholar
PubMed
G-J. Chang
G-J. Chang
Center for Infectious Diseases, Centers for Disease Control, National Institute of Virology, Fort Collins, Colorado, India
Search for other papers by G-J. Chang in
Current site
Google Scholar
PubMed
Search for other papers by G-J. Chang in
Current site
Google Scholar
PubMed
B. Sheik
B. Sheik
Center for Infectious Diseases, Centers for Disease Control, National Institute of Virology, Fort Collins, Colorado, India
Search for other papers by B. Sheik in
Current site
Google Scholar
PubMed
Search for other papers by B. Sheik in
Current site
Google Scholar
PubMed
R. A. Bolin
R. A. Bolin
Center for Infectious Diseases, Centers for Disease Control, National Institute of Virology, Fort Collins, Colorado, India
Search for other papers by R. A. Bolin in
Current site
Google Scholar
PubMed
Search for other papers by R. A. Bolin in
Current site
Google Scholar
PubMed
T. F. Tsai
T. F. Tsai
Center for Infectious Diseases, Centers for Disease Control, National Institute of Virology, Fort Collins, Colorado, India
Search for other papers by T. F. Tsai in
Current site
Google Scholar
PubMed
Search for other papers by T. F. Tsai in
Current site
Google Scholar
PubMed
D. W. Trent
D. W. Trent
Center for Infectious Diseases, Centers for Disease Control, National Institute of Virology, Fort Collins, Colorado, India
Search for other papers by D. W. Trent in
Current site
Google Scholar
PubMed
Search for other papers by D. W. Trent in
Current site
Google Scholar
PubMed
We have isolated and characterized 3 monoclonal antibody (Mab) reagents useful in the serological identification of varieties of eastern equine encephalitis (EEE) viruses. These antibodies were specific for the E1 glycoprotein of their homologous viruses. One Mab, 1B5C-3, reacted specifically with all North American (NA) EEE viruses isolated over a 50 year period. This antigenic stability of NA isolates was genetically confirmed by oligonucleotide fingerprinting. Evolutionary stability is a unique feature among alphaviruses. The Mab 1C1J-4 reacted specifically with 1 South American isolate of EEE virus. A third Mab, 1B1C-4, was EEE virus complex reactive. While none of these antibodies had virus neutralizing activity, the identified reactivities could be demonstrated in the more rapid serological tests of enzyme-linked immunosorbent assay and indirect immunofluorescence.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 876 | 555 | 24 |
| Full Text Views | 54 | 21 | 0 |
| PDF Downloads | 47 | 14 | 0 |