Use of Synthetic Peptides in the Study of the Antibody Response to Plasmodium Vivax Sporozoites

Antonello PessiWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Martine MichelWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Elisabetta BianchiWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Asoka MendisWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Chantal TougneWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Antonio S. VerdiniWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Paul-Henri LambertWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Richard CarterWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Kamini MendisWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Giuseppe del GiudiceWHO-Immunology Research and Training Center, University of Geneva, Sclavo, Monterotondo, University of Colombo, University of Edinburgh, Geneva, Switzerland

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Synthetic peptides reproducing 4 DRADGQPAG (D4) and a sequential array of DRADGQPAG and DRAAGQPAG repeats (DDAAD) of the Plasmodium vivax circumsporozoite (CS) protein were investigated for their potential use in the detection of P. vivax sporozoite antibodies in human sera. These peptides specifically inhibited the binding of monoclonal antibodies to the P. vivax CS protein in Western blots. However, when D4 and DDAAD peptides were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of human antibodies, more sera bound to the DDAAD (61%) than to the D4 peptide (22%). This binding was specific, and suggested that the DDAAD peptide contained epitopes constituted by the sequential array of DRADGQPAG and DRAAGQPAG repeat variants and absent in the D4 peptide. The ELISA using the DDAAD peptide was applied to the detection of P. vivax CS protein antibodies in a large number of sera from Kataragama, an endemic area in Sri Lanka. The prevalence of these antibodies increased with age, reaching 40% in adults > 50 years old. The ELISA employing the DDAAD peptide represents a simple and useful tool for the analysis of the antibody response to P. vivax sporozoites in naturally exposed individuals.

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