Monoclonal Antibodies for Dengue Virus prM Glycoprotein Protect Mice against Lethal Dengue Infection

B. M. KaufmanWalter Reed Army Institute of Research, Washington, DC

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P. L. SummersWalter Reed Army Institute of Research, Washington, DC

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D. R. DuboisWalter Reed Army Institute of Research, Washington, DC

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W. Houston CohenWalter Reed Army Institute of Research, Washington, DC

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M. K. GentryWalter Reed Army Institute of Research, Washington, DC

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R. L. TimchakWalter Reed Army Institute of Research, Washington, DC

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D. S. BurkeWalter Reed Army Institute of Research, Washington, DC

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K. H. EckelsWalter Reed Army Institute of Research, Washington, DC

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Five murine monoclonal antibodies (Mabs) reactive against the prM glycoproteins of DEN-3 and -4 were used to passively protect mice in vivo against lethal challenge with homologous and heterologous dengue virus serotypes. Four of the 5 prM-reactive monoclonals cross-protected mice against heterologous challenge, whereas 1 protected against challenge with only the homologous serotype. Although in vitro binding to virions was readily demonstrated, only 2 of the prM Mabs had detectable neutralizing activity. The neutralizing activity could not be enhanced by anti-mouse immunoglobulin or complement. However, 4 of the 5 prM Mabs fixed complement. This is the first report of prM-specific Mabs that are protective in mice.

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