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Isoenzyme Analysis of Human and Animal Isolates of Giardia Duodenalis from British Columbia, Canada
Eileen M. Proctor
Eileen M. Proctor
British Columbia Ministry of Health, University of British Columbia, Vancouver, British Columbia, Canada
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Judith L. Isaac-Renton
Judith L. Isaac-Renton
British Columbia Ministry of Health, University of British Columbia, Vancouver, British Columbia, Canada
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Janet Boyd
Janet Boyd
British Columbia Ministry of Health, University of British Columbia, Vancouver, British Columbia, Canada
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Quantine Wong
Quantine Wong
British Columbia Ministry of Health, University of British Columbia, Vancouver, British Columbia, Canada
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William R. Bowie
William R. Bowie
British Columbia Ministry of Health, University of British Columbia, Vancouver, British Columbia, Canada
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Isoenzyme patterns of 32 isolates of Giardia duodenalis, obtained from 6 beavers and 11 humans from British Columbia, plus 15 other isolates were evaluated using thin-layer starch-gel electrophoresis. We attempted to use 12 enzymes; 9 gave reproducible and interpretable results. The isoenzyme patterns of the isolates were classified into 12 groups with 17 (53%) of the 32 isolates confined to 1 group. The other 11 groups each comprised only 1 or 2 isolates. There was no obvious correlation between clinical symptoms and isoenzyme patterns. Our findings suggest that beavers, like humans and gerbils are receptive to organisms with many different isoenzyme patterns.
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