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Long-Term Cultured Human Vascular Endothelial Cells (EC-FP5) Bind Plasmodium Falciparum Infected Erythrocytes
I. J. Udeinya
I. J. Udeinya
Walter Reed Army Institute of Research, Washington, DC
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C. Magowan
C. Magowan
Walter Reed Army Institute of Research, Washington, DC
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J. D. Chulay
J. D. Chulay
Walter Reed Army Institute of Research, Washington, DC
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We have established an endothelial cell line, EC-EP5, that binds Plasmodium falciparum infected erythrocytes after several passages in culture and multiple cryopreservations. Binding by the EC-FP5 cells, measured as the percentage that bound infected erythrocytes and the average number of infected erythrocytes bound per endothelial cell, was similar in cells cryopreserved 1, 2, or 3 times. The parasite strain of the infected erythrocytes and culture conditions, including parasitemia and pH of the erythrocyte suspension, significantly affected binding. The capability of the EC-FP5 cells to be cultured in large amounts and cryopreserved in several aliquots will provide flexibility, reduce experimental variation, and enhance the utility of the endothelial cell-dependent cytoadherence assay.
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| Past two years | Past Year | Past 30 Days | |
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