Sensitive Determination of Circulating Anodic Antigen in Schistosoma mansoni Infected Individuals by an Enzyme-Linked Immunosorbent Assay using Monoclonal Antibodies

André M. DeelderLaboratory of Parasitology, State University of Leiden, The Netherlands

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Niels De JongeLaboratory of Parasitology, State University of Leiden, The Netherlands

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Otto C. BoermanLaboratory of Parasitology, State University of Leiden, The Netherlands

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Yvonne E. FilliéLaboratory of Parasitology, State University of Leiden, The Netherlands

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Gerard W. HilberathLaboratory of Parasitology, State University of Leiden, The Netherlands

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J. Peter RotmansLaboratory of Parasitology, State University of Leiden, The Netherlands

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Marijke J. GerritseLaboratory of Parasitology, State University of Leiden, The Netherlands

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D. W. O. Lex SchutLaboratory of Parasitology, State University of Leiden, The Netherlands

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From a panel of mouse monoclonal antibodies reactive with a repeating epitope of the schistosome circulating anodic antigen, an IgG1 monoclonal antibody was selected. This monoclonal antibody was applied in a sandwich enzyme-linked immunosorbent assay as capture antibody and as alkaline phosphatase labeled conjugate.

This assay allowed a sensitive quantitation of circulating anodic antigen in serum samples of infected individuals, detecting < 1 ng antigen/ml serum.

In Schistosoma mansoni infected individuals from Zaire, the level of antigen in serum correlated with fecal egg output. The lower detection level of the immunoassay corresponded to a level of about 10 eggs/gm feces.

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