Identification, Using Isoenzyme Electrophoresis and Monoclonal Antibodies, of Leishmania Isolated from Humans and Wild Animals of Ecuador

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  • Department of Parasitic Diseases, Kumamoto University Medical School, Department of Immunology, Instituto Oswaldo Cruz, Rio de Janeiro, Biology Department Youngstown State University, Departamento de Parasitologia, Instituto Nacional de Higiene y Medicina Tropical, Department of Epidemiology and Public Health, Yale University School of Medicine, Department of Parasitology, Kochi Medical School, Kumamoto, Japan
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Six strains of Leishmania isolated from wild mammals and humans on the Pacific Coast of Ecuador were identified by isoenzyme electrophoresis and by their reactivity patterns to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. Single isolates from Sciurus vulgaris. Potos flavus, and Tamandua tetradactyla were identified as Leishmania amazonensis. Three other strains, isolated from cutaneous lesions of humans, were identified as Leishmania panamensis.