Quantitative Determination of Circulating Antigens in Human Schistosomiasis Mansoni using an Indirect Hemagglutination Assay

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  • 1 Laboratory of Parasitology, Medical Faculty, State University of Leiden, Wassenaarseweg 62, Leiden, The Netherlands
  • | * Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China
  • | Service Médical, Société Minière et Industrielle du Kivu, Kalima, Zaire
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In serum and urine specimens collected from a group of Schistosoma mansoni infected individuals from Makundju, Zaire, the schistosome circulating anodic antigen (CAA) and the circulating cathodic antigen (CCA) were quantitatively determined using an indirect hemagglutination reaction with sheep erythrocytes sensitized with mouse IgM monoclonal antibodies directed against these circulating antigens. Levels of CAA in serum (up to 5 ng/ml) and CCA in serum and urine (up to 50 ng/ml) were strongly correlated with egg excretion and with each other. No correlation was found between egg excretion and antibody levels against the circulating antigens. Antigen was detectable only in patients excreting > 500 eggs per gram of feces.