Presence of Antibodies to the Major Surface Glycoprotein of Trypanosoma cruzi Amastigotes in sera from Chagasic Patients

Norma W. AndrewsDepartment of Pathology and Kaplan Cancer Center, New York University Medical Center, New York, New York 10016

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Monica EinsteinDepartment of Pathology and Kaplan Cancer Center, New York University Medical Center, New York, New York 10016

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Victor NussenzweigDepartment of Pathology and Kaplan Cancer Center, New York University Medical Center, New York, New York 10016

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The surface of amastigote forms of Trypanosoma cruzi is covered by a stage-specific glycoprotein, Ssp-4. We show that Y strain-derived Ssp-4 is recognized by antibodies in sera from Chagasic patients. All 51 sera reacted with the surface of amastigotes by indirect immunofluorescence assays and immunoprecipitated Ssp-4. The human antibodies inhibited the binding of monoclonal antibodies to Ssp-4 in immunoradiometric assays, suggesting that the corresponding region of the molecule may be conserved among distinct strains of the parasite.

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