Comparison of Thick Films, in Vitro Culture and DNA Hybridization Probes for Detecting Plasmodium falciparum Malaria

David E. Lanar
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Gerald L. McLaughlinUniversity of Illinois, Urbana, Illinois 61801

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Dyann F. WirthHarvard School of Public Health, Cambridge, Massachusetts 02115

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Robert J. BarkerHarvard School of Public Health, Cambridge, Massachusetts 02115

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J. Werner ZolgBiomedical Research Institute, Bethesda, Maryland 20852

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Jeffrey D. Chulay
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Using blood from volunteers with sporozoite induced malaria, a comparison was made of the sensitivity and specificity of Giemsa stained thick film examination, in vitro culture, and 4 different DNA probes for detecting parasitemia. Between 9 and 13 days after sporozoite inoculation, patent parasitemia (4–550 parasites/µl) was detected by thick film examination of 0.5 µl blood in 7 volunteers. Cultures of 1 ml blood obtained 7 days after sporozoite inoculation were positive in all volunteers who eventually developed patent parasitemia. The DNA hybridization probes detected parasites in only 5–28% of smear- or culture-positive samples.

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