Cultivation of Exoerythrocytic Stages of Plasmodium Cynomolgi, P. Knowlesi, P. Coatneyi, and P. Inui in Macaca Mulatta Hepatocytes

Pascal Millet
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Tamara L. Fisk
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William E. Collins
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J. Roger BrodersonMalaria Branch, Division of Parasitic Diseases

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Phuc Nguyen-Dinh
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Exoerythrocytic stage parasites of Plasmodium cynomolgi, P. knowlesi, P. coatneyi, and P. inui were cultured by inoculating primary cultures of hepatocytes from Macaca mulatta with sporozoites. Less than 1% of inoculated sporozoites survived. Morphology and size of the liver stages in all 4 species were similar to in vivo descriptions and the time required for in vitro maturation correlated well with prepatent periods described.

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