Microinjection of DNA into Aedes Triseriatus Ova and Detection of Integration

View More View Less
  • 1 Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523
  • | * Division of Vector-borne Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522
Restricted access

A technique was developed for microinjection and transformation of Aedes triseriatus mosquitoes. Mosquito ova can be readily microinjected with approximately 0.9 nl of inoculum if desiccated and inoculated before hardening of the chorion. Ova were injected with molecular constructs that conferred antibiotic (G418) resistance to larvae. Insertion of the antibiotic resistance construct into the mosquito genome was demonstrated by Southern blot analysis.