The Development of Nonradioactive Total Genomic Probes for Strain and Egg Differentiation in Taeniid Cestodes

Kok W. Yap Division of Veterinary Biology and the W.H.O. Collaborating Centre for Echinococcosis/Hydatidosis, School of Veterinary Studies, Murdoch University, Murdoch, Western Australia, 6150

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R. C. Andrew Thompson Division of Veterinary Biology and the W.H.O. Collaborating Centre for Echinococcosis/Hydatidosis, School of Veterinary Studies, Murdoch University, Murdoch, Western Australia, 6150

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Izabella D. Pawlowski Division of Veterinary Biology and the W.H.O. Collaborating Centre for Echinococcosis/Hydatidosis, School of Veterinary Studies, Murdoch University, Murdoch, Western Australia, 6150

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DOI:
https://doi.org/10.4269/ajtmh.1988.39.472
Volume/Issue:
Volume 39: Issue 5
Page(s):
472–477
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Purified total genomic DNA labeled with photobiotin was used as a probe for the identification and differentiation of strains of Echinococcus granulosus and to distinguish between the morphologically identical eggs of 2 taeniid species. Such biotinylated probes are easily prepared and can be utilized effectively for rapid screening and identification of taeniid isolates by dot and Southern blotting. The use of total genomic DNA probes in high stringency hybridizations obviates the need for cloned sequences and has great potential for field use.

Author Notes

Copyright:
Copyright © 1988 by The American Society of Tropical Medicine and Hygiene 1988
Accepted:
04 May 1988
|
Published Online:
Nov 1988
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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