The Hypersensitivity Response to the Adult Worm Proteinase, SMw32, in Schistosoma Mansoni Infected Mice

Cynthia L. Chappell
Search for other papers by Cynthia L. Chappell in
Current site
Google Scholar
PubMed
Close
,
D. Chester Kalter Department of Dermatology, Baylor College of Medicine, Houston, Texas 77030

Search for other papers by D. Chester Kalter in
Current site
Google Scholar
PubMed
Close
, and
Marc H. Dresden
Search for other papers by Marc H. Dresden in
Current site
Google Scholar
PubMed
Close
Restricted access

A cysteine proteinase (SMw32) from the digestive tract of an adult Schistosoma mansoni worm has previously been purified and characterized. During the course of infection with this parasite, a strong immune response to the enzyme occurs. We now have confirmed the presence of anti-proteinase IgE and IgG in S. mansoni infected mice and have investigated the in vivo cellular response to proteinase in infected and uninfected mice. Immediate and delayed type hypersensitivities were detected in uninfected mice sensitized by multiple injections of proteinase. In S. mansoni infected mice, immediate hypersensitivity reactions were seen at 6 and 8 weeks following infection, coincident with the increase in anti-proteinase IgE antibody. Histological sections of the injection site confirmed the presence of degranulating mast cells. In contrast, delayed type hypersensitivity could not be detected at any time during the course of the infection. In the murine model of acute infection, immediate hypersensitivity to the SMw32 proteinase was predictive of infection.

Author Notes

Save