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Detection of Leishmania within Sand Flies by Kinetoplast DNA Hybridization
William O. Rogers
William O. Rogers
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Paulo F. Burnheim
Paulo F. Burnheim
Instituto de Medicina Tropical de Manaus, Avenida Dom Pedro Texeira, Manaus, Amazonas, Brazil
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Dyann F. Wirth
Dyann F. Wirth
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A kinetoplast DNA hybridization probe method was used to detect Leishmania within sand flies and to distinguish it from the non-pathogenic flagellate, Endotrypanum. Eighty-one sand flies (74 Lutzomyia umbratilis, 1 Lu. anduzei, and 6 Lu. shannoni) collected outside Manaus, Brazil were dissected. Forty-four of these were found to be infected with flagellates, and 2 hybridized with a Leishmania braziliensis probe. Thirty-three of sixty-one flies reprobed with an Endotrypanum probe were positive.
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| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 447 | 185 | 77 |
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