In Vitro Cultivation of Exoerythrocytic Stages of the Human Malaria Parasite Plasmodium Malariae

Pascal MilletMalaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

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William E. CollinsMalaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

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Tamara L. FiskMalaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

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Phuc Nguyen-DinhMalaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

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Exoerythrocytic stage parasites of Plasmodium malariae were obtained in vitro by inoculating primary cultures of hepatocytes from a chimpanzee (Pan troglodytes) and a monkey (Aotus lemurinus griseimembra) with sporozoites. Schizonts were observed in chimpanzee hepatocytes 8, 11, and 13 days after inoculation. Only 1 schizont was seen in Aotus hepatocytes at day 13. The morphology and development rates of P. malariae exoerythrocytic stages obtained in vitro were similar to those previously described in vivo.

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