In Vitro Cultivation of Exoerythrocytic Stages of the Human Malaria Parasite Plasmodium Malariae

Pascal Millet Malaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

Search for other papers by Pascal Millet in
Current site
Google Scholar
PubMed
Close
,
William E. Collins Malaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

Search for other papers by William E. Collins in
Current site
Google Scholar
PubMed
Close
,
Tamara L. Fisk Malaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

Search for other papers by Tamara L. Fisk in
Current site
Google Scholar
PubMed
Close
, and
Phuc Nguyen-Dinh Malaria Branch, Division of Parasitic Diseases Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

Search for other papers by Phuc Nguyen-Dinh in
Current site
Google Scholar
PubMed
Close
Restricted access

Exoerythrocytic stage parasites of Plasmodium malariae were obtained in vitro by inoculating primary cultures of hepatocytes from a chimpanzee (Pan troglodytes) and a monkey (Aotus lemurinus griseimembra) with sporozoites. Schizonts were observed in chimpanzee hepatocytes 8, 11, and 13 days after inoculation. Only 1 schizont was seen in Aotus hepatocytes at day 13. The morphology and development rates of P. malariae exoerythrocytic stages obtained in vitro were similar to those previously described in vivo.

Author Notes

Save