Uptake of Antibiotics by Plasmodium falciparum in Culture

Timothy G. Geary Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824-1101

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Alan A. Divo Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824-1101

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James B. Jensen Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824-1101

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DOI:
https://doi.org/10.4269/ajtmh.1988.38.466
Volume/Issue:
Volume 38: Issue 3
Page(s):
466–469
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The ability of Plasmodium falciparum to accumulate 3H-clindamycin, 3H-tetracycline, and 3H-dihydrostreptomycin was determined in synchronized trophozoites and schizonts in 87% parasitemia 5% hematocrit cultures. Accumulation was time-dependent and modestly concentration-dependent. At 0.1 mM initial external drug concentration, the ratio of drug concentration in cells:medium after 120 min was 5.5 for 3H-clindamycin, 2.8 for 3H-tetracycline, and 0.95 for 3H-dihydrostreptomycin. Corresponding values for uninfected erythrocytes were 3.2, 2.3, and 0.78, respectively. These data may explain the lack of antimalarial activity of the aminoglycosides and may partially explain the slow onset of therapeutic action of clindamycin and tetracycline. The low intracellular concentrations attained for the latter two drugs have important implications for understanding the kinetics and mechanism of their antiplasmodial effects.
Copyright:
Copyright © 1988 by The American Society of Tropical Medicine and Hygiene 1988
Accepted:
23 Nov 1987
|
Published Online:
May 1988
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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