Ecology of Viruses Isolated from Sand Flies in Italy and Characterization of a New Phlebovirus (Arbia Virus)

Paola VeraniLaboratory of Virology, Istituto Superiore di Sanità, Rome

Search for other papers by Paola Verani in
Current site
Google Scholar
PubMed
Close
,
Maria Grazia CiufoliniLaboratory of Virology, Istituto Superiore di Sanità, Rome

Search for other papers by Maria Grazia Ciufolini in
Current site
Google Scholar
PubMed
Close
,
Silvana CaciolliLaboratory of Virology, Istituto Superiore di Sanità, Rome

Search for other papers by Silvana Caciolli in
Current site
Google Scholar
PubMed
Close
,
Antonella RenziLaboratory of Virology, Istituto Superiore di Sanità, Rome

Search for other papers by Antonella Renzi in
Current site
Google Scholar
PubMed
Close
,
Loredana NicolettiLaboratory of Virology, Istituto Superiore di Sanità, Rome

Search for other papers by Loredana Nicoletti in
Current site
Google Scholar
PubMed
Close
,
Guido SabatinelliInstitute of Parasitology, “La Sapienza,” University of Rome

Search for other papers by Guido Sabatinelli in
Current site
Google Scholar
PubMed
Close
,
Dario BartolozziDepartment of Infectious Diseases, S. Maria Nuova Hospital, Florence

Search for other papers by Dario Bartolozzi in
Current site
Google Scholar
PubMed
Close
,
Gino VolpiDepartment of Neurology, University of Florence

Search for other papers by Gino Volpi in
Current site
Google Scholar
PubMed
Close
,
Luigi AmaducciDepartment of Neurology, University of Florence

Search for other papers by Luigi Amaducci in
Current site
Google Scholar
PubMed
Close
,
Mario ColuzziInstitute of Parasitology, “La Sapienza,” University of Rome

Search for other papers by Mario Coluzzi in
Current site
Google Scholar
PubMed
Close
,
Pietro PaciDepartment of Infectious Diseases, S. Maria Nuova Hospital, Florence

Search for other papers by Pietro Paci in
Current site
Google Scholar
PubMed
Close
, and
Marco BalducciEpidemiological Investigation Unit for Neurotropic Viruses, Toscana Region, Florence, Italy

Search for other papers by Marco Balducci in
Current site
Google Scholar
PubMed
Close
Restricted access

A total of 84 virus strains was obtained from 16,374 male and female sand flies (Phlebotomus perniciosus and P. perfiliewi) collected in two localities of Tuscany region in Italy between 1980 and 1985. Thirty-seven (44%) were identified as Toscana virus (family Bunyaviridae, genus Phlebovirus) and 47 (56%) as a new member of the Phlebotomus fever serogroup, Arbia virus. The characteristics of this new serotype are described. The overall virus isolation rate from sand flies was 0.5 per 100 insects processed. Virus isolation rates for both viruses were similar in different years and in the two localities, suggesting that the two virus types were active in the sand fly population simultaneously. Each year, the largest number of isolates were obtained during July, corresponding to the period of maximal sand fly population density. Both viruses were repeatedly isolated from male sand flies, suggesting transovrial transmission in nature.

Serologic data showed no evidence of infection among domestic and wild animals. However, a strain of Toscana virus was isolated from the brain of a bat (Pipistrellus kuhli), indicating a possible involvement of this species in the ecology of the virus. Serologic tests did not provide definitive evidence for human infection by Arbia virus.

Save