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The Use of Nonradioactive DNA Probes for the Characterization of Leishmania Isolates from Peru
Martin López
Martin López
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Ysabel Montoya
Ysabel Montoya
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Margarita Arana
Margarita Arana
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Francisco Cruzalegui
Francisco Cruzalegui
Instituto de Medicina Tropical “Alexander von Humboldt,”
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Janet Braga
Janet Braga
Universidad Nacional de la Amazonía
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Alejandro Llanos-Cuentas
Alejandro Llanos-Cuentas
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Guillermo Romero
Guillermo Romero
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Jorge Arévalo
Jorge Arévalo
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We describe conditions for dot blot DNA hybridization studies using biotinylated kDNA probes from Leishmania. The sensitivity and specificity attained with biotinylated or 32P-labeled probes were equivalent. The lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with Proteinase K. Studies were performed with 112 Leishmania isolates from Andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the Leishmania braziliensis complex.
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| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 2019 | 1630 | 62 |
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