Use of Synthetic and Recombinant Peptides in the Study of Host-Parasite Interactions in the Malarias

G. H. Campbell Malaria Branch and Parasitic Diseases Branch, Division of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia

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S. B. Aley Malaria Department, Biomedical Research Institute, Rockville, Maryland

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W. R. Ballou Department of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

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T. Hall Department of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

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W. T. Hockmeyer Department of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

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S. L. Hoffman

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M. R. Hollingdale Malaria Department, Biomedical Research Institute, Rockville, Maryland

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R. J. Howard Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

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J. A. Lyon Department of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

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E. H. Nardin Department of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

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R. S. Nussenzweig Department of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

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V. Nussenzweig Department of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

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V. C. W. Tsang Malaria Branch and Parasitic Diseases Branch, Division of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia

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J. L. Weber Department of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

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T. E. Wellems Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

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J. F. Young Molecular Genetics, Smith, Kline and French Laboratories, Swedeland, Pennsylvania

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F. Zavala Department of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

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DOI:
https://doi.org/10.4269/ajtmh.1987.37.428
Volume/Issue:
Volume 37: Issue 3
Page(s):
428–444
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INTRODUCTION* Recombinant and synthetic peptide technology has allowed new approaches to the study of parasitic diseases. A major benefit of these advances is the ease with which entire genes can be isolated and sequenced at the nucleotide level. This information can help answer questions on genomic organization and evolution. Moreover, we can deduce the entire amino acid sequence of a particular protein without having sufficient protein to visualize on an acrylamide gel. The challenge of translating the nucleotide sequence into a biologically relevant and functional protein remains.

We intend to focus attention on a particular way to accomplish that translation, i.e., the use of polypeptides derived from the deduced sequence to probe or model a function or property of the protein. It is often easier to obtain large amounts of relatively pure recombinant or synthetic polypeptides than of the original protein. Caveats about the use of such peptides cannot be overstated.

Author Notes

Copyright:
Copyright © 1987 by The American Society of Tropical Medicine and Hygiene 1987
Published Online:
Nov 1987
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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