Use of Synthetic and Recombinant Peptides in the Study of Host-Parasite Interactions in the Malarias

G. H. CampbellMalaria Branch and Parasitic Diseases Branch, Division of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia

Search for other papers by G. H. Campbell in
Current site
Google Scholar
PubMed
Close
,
S. B. AleyMalaria Department, Biomedical Research Institute, Rockville, Maryland

Search for other papers by S. B. Aley in
Current site
Google Scholar
PubMed
Close
,
W. R. BallouDepartment of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

Search for other papers by W. R. Ballou in
Current site
Google Scholar
PubMed
Close
,
T. HallDepartment of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

Search for other papers by T. Hall in
Current site
Google Scholar
PubMed
Close
,
W. T. HockmeyerDepartment of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

Search for other papers by W. T. Hockmeyer in
Current site
Google Scholar
PubMed
Close
,
S. L. Hoffman

Search for other papers by S. L. Hoffman in
Current site
Google Scholar
PubMed
Close
,
M. R. HollingdaleMalaria Department, Biomedical Research Institute, Rockville, Maryland

Search for other papers by M. R. Hollingdale in
Current site
Google Scholar
PubMed
Close
,
R. J. HowardLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Search for other papers by R. J. Howard in
Current site
Google Scholar
PubMed
Close
,
J. A. LyonDepartment of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

Search for other papers by J. A. Lyon in
Current site
Google Scholar
PubMed
Close
,
E. H. NardinDepartment of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

Search for other papers by E. H. Nardin in
Current site
Google Scholar
PubMed
Close
,
R. S. NussenzweigDepartment of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

Search for other papers by R. S. Nussenzweig in
Current site
Google Scholar
PubMed
Close
,
V. NussenzweigDepartment of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

Search for other papers by V. Nussenzweig in
Current site
Google Scholar
PubMed
Close
,
V. C. W. TsangMalaria Branch and Parasitic Diseases Branch, Division of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia

Search for other papers by V. C. W. Tsang in
Current site
Google Scholar
PubMed
Close
,
J. L. WeberDepartment of Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC

Search for other papers by J. L. Weber in
Current site
Google Scholar
PubMed
Close
,
T. E. WellemsLaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Search for other papers by T. E. Wellems in
Current site
Google Scholar
PubMed
Close
,
J. F. YoungMolecular Genetics, Smith, Kline and French Laboratories, Swedeland, Pennsylvania

Search for other papers by J. F. Young in
Current site
Google Scholar
PubMed
Close
, and
F. ZavalaDepartment of Medical and Molecular Parasitology and Department of Pathology, New York University, New York, New York

Search for other papers by F. Zavala in
Current site
Google Scholar
PubMed
Close
Restricted access

INTRODUCTION Recombinant and synthetic peptide technology has allowed new approaches to the study of parasitic diseases. A major benefit of these advances is the ease with which entire genes can be isolated and sequenced at the nucleotide level. This information can help answer questions on genomic organization and evolution. Moreover, we can deduce the entire amino acid sequence of a particular protein without having sufficient protein to visualize on an acrylamide gel. The challenge of translating the nucleotide sequence into a biologically relevant and functional protein remains.

We intend to focus attention on a particular way to accomplish that translation, i.e., the use of polypeptides derived from the deduced sequence to probe or model a function or property of the protein. It is often easier to obtain large amounts of relatively pure recombinant or synthetic polypeptides than of the original protein. Caveats about the use of such peptides cannot be overstated.

Save