- Materials and Methods
- Results
- Screening of O. volvulus (savanna) genomic library for highly repeated DNA sequences
- Hybridization of puOvs3 to different geographic isolates of O. volvulus and to other Onchocerca species
- Relative abundance of repeated sequence puOvs3 in the genome of O. volvulus
- Sensitivity of clone puOvs3 as a diagnostic probe
- Restriction map of plasmid puOvs3
- Sequence of puOvs3
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Duke B. O. L. 1972. Onchocerciasis. Br. Med. Bull., 28 :66–71
-
2.
Duke B. O. L. 1967. Infective filarial larvae, other than Onchocerca volvulus, in Simulium damnosum. Trap. Med. Parasit., 61 :200–205
-
3.
Garms R. & Voelker J. 1969. Unknown filarial larvae and zoophily in Simulium damnosum in Liberia. Trans. R. Soc. Trap. Med. Hyg., 63 :676–677
-
4.
Garms R. 1983. Studies of the transmission of Onchocerca volvulus by species of the Simulium damnosum complex occurring in Liberia. Z. Angew. Zool., 70 :101–117
-
5.
Nelson G. S. & Pester F. R. N. 1962. The identification of infective larvae in Simuliidae. Bull. W.H.O., 27 :473–481
-
6.
Garms R. 1985. Morphological differentiation from Onchocerca volvulus of first stage larvae of an unknown filarial species commonly found in Simulium sanctipauli s.l. in Liberia. Trap. Med. Parasit., 36 :255–256
-
7.
Omar M.S., Denke A. M. & Raybould J. N. 1979. The development of O. ochengi to the infective stage in Simulium damnosum s.l.. Tropenmed. Parasit., 30 :157–162
-
8.
Walsh J. F., Davies J. B., Le Berre & Garms R. 1978. Standardization of criteria for assessing the effect of Simulium control in onchocerciasis control programme. Trans. R. Soc. Trap. Med. Hyg., 72 :675–676
-
9.
Nelson G. S. 1970. Onchocerciasis. Pages 173–224 inDaves B., ed., Advances in Parasitology, Volume 8. Academic Press, London and New York.
-
10.
Duke B. O. L. 1976. Strains of Onchocerca volvulus and their pathogenicity. Tropenmed. Parasitol., 27 :21–22
-
11.
Duke B. O. L., Lewis D. J. & Moore P. J. 1966. Onchocerca-simulium complexes. I. Transmission of forest and Sudan savanna strains of Onchocerca volvulus from Cameroon by Simulium damnosum of various West African bioclimatic zones. Ann. Trop. Med. Parasit., 60 :318–335
-
12.
Lewis D. J. & Duke B. O. L. 1966. Onchocerca-Simulium complexes. II. Variation in West African female Simulium damnosum. Ann. Trap. Med. Parasitol., 60 :337–346
-
13.
De Leon J. R. & Duke B. O. L. 1966. Experimental studies on the transmission of Guatemalan and West African strains of O. volvulus by Simulium ochraceum, S. metallicum and S. callidum. Trans. R. Soc. Trap. Med. Hyg., 60 :735–752
-
14.
Duke B. O. L. 1967. Onchocerca-Simulium complexes. IV. Transmission of a variant of a forest strain of Onchocerca volvulus. Ann. Trap. Med. Parasit., 61 :326–331
-
15.
Duke B. O. L., Moore P. J. & De Leon J. R. 1967. Onchocerca-Simulium complexes. V. The intake and subsequent fate of microfilariae of a Guatemalan strain of Onchocerca volvulus in forest and Sudan savanna forms of the West African Simulium damnosum. Ann. Trap. Med. Parasit., 61 :332–337
-
16.
Duke B. O. L. 1970. Onchocerca-Simulium complexes. VI. Experimental studies on the transmission of Venezuelan and West African strains of Onchocerca volvulus by Simulium metallicum and S. exiguum in Venezuela. Ann. Trap. Med. Parasit., 64 :421–431
-
17.
Budden F. H. 1963. Comparative study of occular onchocerciasis in savanna and rain forest. Trans. R. Soc. Trap. Med. Hyg., 57 :64–70
-
18.
Anderson J., Fuglsang H., Hamilton P. J. S. & de C. Marshall T. F. 1974. Studies on onchocerciasis in the United Cameroon Republic. II. Comparison of onchocerciasis in rain forest and Sudan savanna. Trans. R. Soc. Trap. Med. Hyg., 68 :209–222
-
19.
Anderson J., Fuglsang H. & de C. Marshall T. F. 1976. Studies on onchocerciasis in the United Cameroon Republic. III. A four year follow-up of 6 rain forest and 6 Sudan savanna villages. Trans. R. Soc. Trap. Med. Hyg., 70 :362–373
-
20.
Prost A., Rougemont A. & Omar M. S. 1980. Caracteres epidemiologiques, cliniques et biologiques des onchocercoses de sa vane et de forêt en Afrique occidentale. Ann. Parasit., 55 :347–355
-
21.
Duke B. O. L. & Anderson J. 1972. A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan savanna strains of Onchocerca volvulus from Cameroon. Z. Tropenmed. Parasit., 23 :354–368
-
22.
Omar M. S. 1978. Histochemical enzyme straining patterns of Onchocerca volvulus micro filariae and their occurrence in different onchocerciasis areas. Tropenmed. Parasit., 29 :462–472
-
23.
Bryceson A. D. M., Schillhorn van Veen K., Oduloju A. J. & Duke B. O. L. 1976. Antigenic diversity among Onchocerca volvulus in Nigeria and immunological differences between onchocerciasis in the savanna and forest of Cameroon. Clin. Exp. Immunol., 24 :168–176
-
24.
Lobos E. & Weiss N. 1985. Immunological comparison between worm extracts of Onchocerca volvulus from savanna and rain forest. Parasite Immunol., 7 :333–347
-
25.
Cianchi R., Karam M., Henry M. C., Villani F., Kumlien S. & Bulhini L. 1985. Preliminary data on the genetic differentiation of Onchocerca volvulus in Africa (Nematoda: Filarioidea). Acta Trap., 42 :341–351
-
26.
Flavel R. B. 1982. Sequence amplification, deletion and rearrangement: Major sources of variation during species divergence. Pages 301–323 inDover G. A., Flavel R. B., eds., Genome Evolution. Academic Press, New York.
-
27.
Wirth D. F. & McMahon-Pratt D. 1982. Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA. Proc. Natl. Acad. Sci. USA, 77 :6999–7003
-
28.
Enea V. 1983. Sensitive and specific DNA probe for detection of Plasmodium falciparum. Mol. Cell. Biol., 6 :321–324
-
29.
Barker R. H. Jr., Suebsaeng H., Rooney W., Alecrim G. C., Dourado H. V. & Wirth D. F. 1986. Specific DNA probe for the diagnosis of Plasmodium falciparum malaria. Science, 231 :434–436
-
30.
McReynolds L. A., De Simone S. M. & Williams S. A. 1986. Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugia malayi and the animal parasite Brugia pahangi. Proc. Natl. Acad. Sci. USA, 83 :797–801
-
31.
Sim B. K. L., Piessens W. F. & Wirth D. F. 1986. A DNA probe cloned in E. coli for the identification of Brugia malayi. Mol. Biochem. Parasitol., 19 :117–123
-
32.
Perter F. B. & Karam M. 1986. Cloning and characterization of two Onchocerca volvulus repeated DNA sequences. Mol. Biochem. Parasit., 21 :171–178
-
33.
Shah J. S., Lamontagne L., Unnasch T. R., Wirth D. F. & Piessens W. F. 1986. Characterization of a ribosomal DNA clone of B. malayi. Mol. Biochem. Parasit., 19 :67–75
-
34.
Schulz-Key H., Albiez E. J. & Buttner D. W. 1977. Isolation of living adult Onchocerca volvulus from nodules. Tropenmed. Parasit., 28 :428–430
-
35.
Unnasch T. R. & Wirth D. F. 1983. The avian malaria Plasmodium/ophurae has a small number of heterogeneous ribosomal RNA genes. Nucl. Acid Res., 11 :8443–8459
-
36.
Maniatis T., Fritsch E. F. & Sambrook J. 1982. Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring, New York.
-
37.
Southern E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Malec. Biol., 98 :503–517
-
38.
Rigby P. W. J., Dickerman M., Rhodes C. & Berg P. 1977. Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase. J. Molec. Biol., 113 :237–251
-
39.
McDonnell M. W., Simon M. N. & Studier F. W. 1977. Analysis of restriction fragment of T7 DNA and determination of molecular weight by electrophoresis in neutral and alkaline gels. J. Molec. Biol., 110 :119–147
-
40.
Sanger F., Nicklen S. & Coulson A. R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA, 74 :5463–5467
-
41.
Long E. O. & David I. B. 1980. Repeated genes in eukaryotes. Ann. Rev. Biochem., 49 :727–764
-
42.
Bain O. 1981. Le genre Onchocerca; hypotheses sur son evolution et cle dichorotique des especes. Ann. Parasit., 56 :503–526
| Past two years | Past Year | Past 30 Days | |
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Characterization of an Onchocerca-Specific DNA Clone from Onchocerca Volvulus
Jyotsna S. Shah
Jyotsna S. Shah
Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115
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Marc Karam
Marc Karam
* Onchocerciasis Control Programme, B.P. 549, Ouagadougou, Burkina Faso
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Willy F. Piessens
Willy F. Piessens
Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115
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Dyann F. Wirth
Dyann F. Wirth
Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115
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A genomic library of a savanna isolate of Onchocerca volvulus was screened to detect recombinant plasm ids containing highly repeated DNA sequences of this parasite. Four recombinant plasmids were identified which hybridized specifically to Onchocerca DNA, but not to DNA from humans, black flies, Brugia malayi, B. pahangi, or Wuchereria bancrofti. The recombinant plasm ids had a low level of homology to Dirofilaria immitis. All recombinant plasmids contain related DNA sequences based on Southern hybridization analysis. Sequences related to these recombinant plasmids are present in different geographic isolates of O. volvulus and O. ochengi, an animal parasite. Two of the recombinant plasmids contain sequences also found in O. lienalis. One recombinant plasmid, puOvs3, has been characterized in detail, including DNA sequence determination. Radiolabeled puOvs3 is able to detect 100 pg of genomic DNA isolated from O. volvulus worms from both savanna and forest regions. It can differentiate O. volvulus from O. ochengi by Southern blot analysis.
Author Notes
Address reprint requests to: Dr. Dyann Wirth, Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115.
- Materials and Methods
- Results
- Screening of O. volvulus (savanna) genomic library for highly repeated DNA sequences
- Hybridization of puOvs3 to different geographic isolates of O. volvulus and to other Onchocerca species
- Relative abundance of repeated sequence puOvs3 in the genome of O. volvulus
- Sensitivity of clone puOvs3 as a diagnostic probe
- Restriction map of plasmid puOvs3
- Sequence of puOvs3
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Duke B. O. L. 1972. Onchocerciasis. Br. Med. Bull., 28 :66–71
-
2.
Duke B. O. L. 1967. Infective filarial larvae, other than Onchocerca volvulus, in Simulium damnosum. Trap. Med. Parasit., 61 :200–205
-
3.
Garms R. & Voelker J. 1969. Unknown filarial larvae and zoophily in Simulium damnosum in Liberia. Trans. R. Soc. Trap. Med. Hyg., 63 :676–677
-
4.
Garms R. 1983. Studies of the transmission of Onchocerca volvulus by species of the Simulium damnosum complex occurring in Liberia. Z. Angew. Zool., 70 :101–117
-
5.
Nelson G. S. & Pester F. R. N. 1962. The identification of infective larvae in Simuliidae. Bull. W.H.O., 27 :473–481
-
6.
Garms R. 1985. Morphological differentiation from Onchocerca volvulus of first stage larvae of an unknown filarial species commonly found in Simulium sanctipauli s.l. in Liberia. Trap. Med. Parasit., 36 :255–256
-
7.
Omar M.S., Denke A. M. & Raybould J. N. 1979. The development of O. ochengi to the infective stage in Simulium damnosum s.l.. Tropenmed. Parasit., 30 :157–162
-
8.
Walsh J. F., Davies J. B., Le Berre & Garms R. 1978. Standardization of criteria for assessing the effect of Simulium control in onchocerciasis control programme. Trans. R. Soc. Trap. Med. Hyg., 72 :675–676
-
9.
Nelson G. S. 1970. Onchocerciasis. Pages 173–224 inDaves B., ed., Advances in Parasitology, Volume 8. Academic Press, London and New York.
-
10.
Duke B. O. L. 1976. Strains of Onchocerca volvulus and their pathogenicity. Tropenmed. Parasitol., 27 :21–22
-
11.
Duke B. O. L., Lewis D. J. & Moore P. J. 1966. Onchocerca-simulium complexes. I. Transmission of forest and Sudan savanna strains of Onchocerca volvulus from Cameroon by Simulium damnosum of various West African bioclimatic zones. Ann. Trop. Med. Parasit., 60 :318–335
-
12.
Lewis D. J. & Duke B. O. L. 1966. Onchocerca-Simulium complexes. II. Variation in West African female Simulium damnosum. Ann. Trap. Med. Parasitol., 60 :337–346
-
13.
De Leon J. R. & Duke B. O. L. 1966. Experimental studies on the transmission of Guatemalan and West African strains of O. volvulus by Simulium ochraceum, S. metallicum and S. callidum. Trans. R. Soc. Trap. Med. Hyg., 60 :735–752
-
14.
Duke B. O. L. 1967. Onchocerca-Simulium complexes. IV. Transmission of a variant of a forest strain of Onchocerca volvulus. Ann. Trap. Med. Parasit., 61 :326–331
-
15.
Duke B. O. L., Moore P. J. & De Leon J. R. 1967. Onchocerca-Simulium complexes. V. The intake and subsequent fate of microfilariae of a Guatemalan strain of Onchocerca volvulus in forest and Sudan savanna forms of the West African Simulium damnosum. Ann. Trap. Med. Parasit., 61 :332–337
-
16.
Duke B. O. L. 1970. Onchocerca-Simulium complexes. VI. Experimental studies on the transmission of Venezuelan and West African strains of Onchocerca volvulus by Simulium metallicum and S. exiguum in Venezuela. Ann. Trap. Med. Parasit., 64 :421–431
-
17.
Budden F. H. 1963. Comparative study of occular onchocerciasis in savanna and rain forest. Trans. R. Soc. Trap. Med. Hyg., 57 :64–70
-
18.
Anderson J., Fuglsang H., Hamilton P. J. S. & de C. Marshall T. F. 1974. Studies on onchocerciasis in the United Cameroon Republic. II. Comparison of onchocerciasis in rain forest and Sudan savanna. Trans. R. Soc. Trap. Med. Hyg., 68 :209–222
-
19.
Anderson J., Fuglsang H. & de C. Marshall T. F. 1976. Studies on onchocerciasis in the United Cameroon Republic. III. A four year follow-up of 6 rain forest and 6 Sudan savanna villages. Trans. R. Soc. Trap. Med. Hyg., 70 :362–373
-
20.
Prost A., Rougemont A. & Omar M. S. 1980. Caracteres epidemiologiques, cliniques et biologiques des onchocercoses de sa vane et de forêt en Afrique occidentale. Ann. Parasit., 55 :347–355
-
21.
Duke B. O. L. & Anderson J. 1972. A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan savanna strains of Onchocerca volvulus from Cameroon. Z. Tropenmed. Parasit., 23 :354–368
-
22.
Omar M. S. 1978. Histochemical enzyme straining patterns of Onchocerca volvulus micro filariae and their occurrence in different onchocerciasis areas. Tropenmed. Parasit., 29 :462–472
-
23.
Bryceson A. D. M., Schillhorn van Veen K., Oduloju A. J. & Duke B. O. L. 1976. Antigenic diversity among Onchocerca volvulus in Nigeria and immunological differences between onchocerciasis in the savanna and forest of Cameroon. Clin. Exp. Immunol., 24 :168–176
-
24.
Lobos E. & Weiss N. 1985. Immunological comparison between worm extracts of Onchocerca volvulus from savanna and rain forest. Parasite Immunol., 7 :333–347
-
25.
Cianchi R., Karam M., Henry M. C., Villani F., Kumlien S. & Bulhini L. 1985. Preliminary data on the genetic differentiation of Onchocerca volvulus in Africa (Nematoda: Filarioidea). Acta Trap., 42 :341–351
-
26.
Flavel R. B. 1982. Sequence amplification, deletion and rearrangement: Major sources of variation during species divergence. Pages 301–323 inDover G. A., Flavel R. B., eds., Genome Evolution. Academic Press, New York.
-
27.
Wirth D. F. & McMahon-Pratt D. 1982. Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA. Proc. Natl. Acad. Sci. USA, 77 :6999–7003
-
28.
Enea V. 1983. Sensitive and specific DNA probe for detection of Plasmodium falciparum. Mol. Cell. Biol., 6 :321–324
-
29.
Barker R. H. Jr., Suebsaeng H., Rooney W., Alecrim G. C., Dourado H. V. & Wirth D. F. 1986. Specific DNA probe for the diagnosis of Plasmodium falciparum malaria. Science, 231 :434–436
-
30.
McReynolds L. A., De Simone S. M. & Williams S. A. 1986. Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugia malayi and the animal parasite Brugia pahangi. Proc. Natl. Acad. Sci. USA, 83 :797–801
-
31.
Sim B. K. L., Piessens W. F. & Wirth D. F. 1986. A DNA probe cloned in E. coli for the identification of Brugia malayi. Mol. Biochem. Parasitol., 19 :117–123
-
32.
Perter F. B. & Karam M. 1986. Cloning and characterization of two Onchocerca volvulus repeated DNA sequences. Mol. Biochem. Parasit., 21 :171–178
-
33.
Shah J. S., Lamontagne L., Unnasch T. R., Wirth D. F. & Piessens W. F. 1986. Characterization of a ribosomal DNA clone of B. malayi. Mol. Biochem. Parasit., 19 :67–75
-
34.
Schulz-Key H., Albiez E. J. & Buttner D. W. 1977. Isolation of living adult Onchocerca volvulus from nodules. Tropenmed. Parasit., 28 :428–430
-
35.
Unnasch T. R. & Wirth D. F. 1983. The avian malaria Plasmodium/ophurae has a small number of heterogeneous ribosomal RNA genes. Nucl. Acid Res., 11 :8443–8459
-
36.
Maniatis T., Fritsch E. F. & Sambrook J. 1982. Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring, New York.
-
37.
Southern E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Malec. Biol., 98 :503–517
-
38.
Rigby P. W. J., Dickerman M., Rhodes C. & Berg P. 1977. Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase. J. Molec. Biol., 113 :237–251
-
39.
McDonnell M. W., Simon M. N. & Studier F. W. 1977. Analysis of restriction fragment of T7 DNA and determination of molecular weight by electrophoresis in neutral and alkaline gels. J. Molec. Biol., 110 :119–147
-
40.
Sanger F., Nicklen S. & Coulson A. R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA, 74 :5463–5467
-
41.
Long E. O. & David I. B. 1980. Repeated genes in eukaryotes. Ann. Rev. Biochem., 49 :727–764
-
42.
Bain O. 1981. Le genre Onchocerca; hypotheses sur son evolution et cle dichorotique des especes. Ann. Parasit., 56 :503–526
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 934 | 614 | 301 |
| Full Text Views | 8 | 1 | 0 |
| PDF Downloads | 14 | 3 | 0 |