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1.
Palmieri J. R., Conner D. H., Pumomo Dennis D. T., Marwoto H. A., 1982. Experimental infection of Wuchereria bancrofti in the silvered leaf monkey Presbytis cristatus Eschscholtz, 1821. J. Helminthol., 56 :243–245.
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2.
Sucharit S., Harinasota C., Choochote W., 1982. Experimental transmission of subperiodic Wuchereria bancrofti to the leaf monkey (Presbytis melalophos), and its periodicity. Am. J. Trap. Med. Hyg., 31 :423–429.
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3.
Cross J. H., Partono F., Hsu M-Y. K., Ash L. R., Oemijati S., 1979. Experimental transmission of Wuchereria bancrofti to monkeys. Am. J. Trap. Med. Hyg., 28 :56–66.
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4.
Mak J. W., Lim P. K. C., Sim B. K. L., Liew L. M., 1983. Brugia malayi and B. pahangi: Cultivation in vitro of infective larvae to the fourth and fifth stages. Exp. Parasitol., 55 :243–248.
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5.
Franke E. D., Weinstein P. P., 1983. Dipetalonema viteae (Nematoda: Filarioidea): Culture of third-stage larvae to young adults in vitro. Science, 221 :161–163.
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6.
Franke E. D., Weinstein P. P., 1984. In vitro cultivation of Dipetalonema viteae third-stage larvae: Effect of the gas phase. J. Parasitol., 70 :493–498.
-
7.
Franke E. D., Weinstein P. P., 1984. In vitro cultivation of Dipetalonema viteae third-stage larvae: Evaluation of culture media, serum and other supplements. J. Parasitol., 70 :618–628.
-
8.
Ash L. R., Schacher J. F., 1971. Early life cycle and larval morphogenesis of Wuchereria bancrofti in the jird, Meriones unguiculatus. J. Parasitol., 57 :1043–1051.
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9.
Lok J. B., Pollack R. J., Cupp E. W., Bernardo M. J., Donnelly J. J., Albiez E. J ., 1984. Development of Onchocerca lienalis and O. volvulus from the third to the fourth larval stage in vitro. Tropenmed. Parasit., 35 :209–211.
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In Vitro Cultivation of Third Stage Larvae of Wuchereria Bancrofti to the Fourth Stage
Eileen D. Franke
Eileen D. Franke
U. S. Naval Medical Research Unit No. 2, Jakarta Detachment, APO San Francisco, California 96356
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Wiwiek Riberu
Wiwiek Riberu
U. S. Naval Medical Research Unit No. 2, Jakarta Detachment, APO San Francisco, California 96356
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Iwa Wiady
Iwa Wiady
U. S. Naval Medical Research Unit No. 2, Jakarta Detachment, APO San Francisco, California 96356
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Third stage larvae of Wuchereria bancrofti obtained from laboratory-infected mosquitoes grew and molted to the fourth stage in vitro. The culture medium which supported the best growth and development consisted of a 1:1 mixture (v/v) of two commercially available cell culture media, NCTC 135 and Iscove's modified Dulbecco's medium supplemented with 10% human serum or plasma and an antibacterial/antimycotic mixture. Cultures were incubated at 37°C in an atmosphere of either 5% or 8% CO2 in air. After 35 days of culture, 65% to 100% of the larvae were fourth stage. They were motile and in excellent morphological condition with development of the reproductive system in males and females. This culture system will provide an important tool for biochemical and immunological studies.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Palmieri J. R., Conner D. H., Pumomo Dennis D. T., Marwoto H. A., 1982. Experimental infection of Wuchereria bancrofti in the silvered leaf monkey Presbytis cristatus Eschscholtz, 1821. J. Helminthol., 56 :243–245.
-
2.
Sucharit S., Harinasota C., Choochote W., 1982. Experimental transmission of subperiodic Wuchereria bancrofti to the leaf monkey (Presbytis melalophos), and its periodicity. Am. J. Trap. Med. Hyg., 31 :423–429.
-
3.
Cross J. H., Partono F., Hsu M-Y. K., Ash L. R., Oemijati S., 1979. Experimental transmission of Wuchereria bancrofti to monkeys. Am. J. Trap. Med. Hyg., 28 :56–66.
-
4.
Mak J. W., Lim P. K. C., Sim B. K. L., Liew L. M., 1983. Brugia malayi and B. pahangi: Cultivation in vitro of infective larvae to the fourth and fifth stages. Exp. Parasitol., 55 :243–248.
-
5.
Franke E. D., Weinstein P. P., 1983. Dipetalonema viteae (Nematoda: Filarioidea): Culture of third-stage larvae to young adults in vitro. Science, 221 :161–163.
-
6.
Franke E. D., Weinstein P. P., 1984. In vitro cultivation of Dipetalonema viteae third-stage larvae: Effect of the gas phase. J. Parasitol., 70 :493–498.
-
7.
Franke E. D., Weinstein P. P., 1984. In vitro cultivation of Dipetalonema viteae third-stage larvae: Evaluation of culture media, serum and other supplements. J. Parasitol., 70 :618–628.
-
8.
Ash L. R., Schacher J. F., 1971. Early life cycle and larval morphogenesis of Wuchereria bancrofti in the jird, Meriones unguiculatus. J. Parasitol., 57 :1043–1051.
-
9.
Lok J. B., Pollack R. J., Cupp E. W., Bernardo M. J., Donnelly J. J., Albiez E. J ., 1984. Development of Onchocerca lienalis and O. volvulus from the third to the fourth larval stage in vitro. Tropenmed. Parasit., 35 :209–211.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 301 | 116 | 3 |
| Full Text Views | 18 | 7 | 0 |
| PDF Downloads | 15 | 6 | 0 |