Immunological Studies in Amebiasis: Isotypic Characterization of Specific Antibodies by Enzyme-Linked Immunofiltration Assay

Jean-Michel Pinon Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 45, rue Cognacq Jay et Rue Alexis Carrel, 51092 Reims Cédex, France

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Jean Poirriez Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 45, rue Cognacq Jay et Rue Alexis Carrel, 51092 Reims Cédex, France

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Gerard Remy *Service de Medécine Interne et des Maladies Infectieuses, Hôpital Robert Debré, Rue Alexis Carrel, 51092 Reims Cédex, France

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Herve Lepan Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 45, rue Cognacq Jay et Rue Alexis Carrel, 51092 Reims Cédex, France

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Fifty sera from 17 cases of invasive amebiasis (15 hepatic localizations, ameboma, 1 diffused colic localization) were studied by enzyme-linked immunofiltration assay (ELIFA). IgM and IgE anti-Entamoeba histolytica were detected in 10 and 15, respectively, of the 17 patients studied. IgM and IgE antibodies were found simultaneously in 10 cases; these 2 isotypes were only recognized twice in the same serum by the same antigenic components. During post-therapy monitoring, in less than 90 days IgE or IgM-Ab regressed completely in half the cases. If they appeared or reappeared after the third month, prognosis was bad. In addition to its value for diagnosis and prognosis, the ELIFA allowed us to detect the functional antigenic components revealed more particularly by some IgG, IgM, IgE, or IgA antibodies.

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