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-
1.
Krupp I. M., 1970. Antibody response in intestinal and extraintestinal amebiasis. Am. J. Trop. Med. Hyg., 19 :57–62.
-
2.
Knobloch J., Mannweiler E., 1983. Development and persistence of antibodies to Entamoeba histolytica in patients with amebic liver abscess. Analysis of 216 cases. Am. J. Trop. Med. Hyg., 32 :727–732.
-
3.
Knae G. J., Matossian R., Batty I., 1971. Fluorochrome-labeled anti-immunoglobulin fractions used with stabilized antigen preparations for the assessment of parasitic diseases. Ann. N.Y. Acad. Sci., 177 :134–145.
-
4.
Capron A., Vernes A., Niel G., Bouvry M., 1972. Le diagnostic immunologique de l'amibiase. Med. Chir. Dig., 1 :5–13.
-
5.
Gentilini M., Pinon J. M., Niel G., 1972. Immunoelectrodiffusion sur membrane d'acétate de cellulose. Applications en parasitologie a propos des résultats préliminaires sur 600 essais. Nouv. Presse Méd., 1 :1499–1501.
-
6.
Krupp I. M., 1974. Comparison of counterimmunoelectrophoresis with other serologic tests in the diagnosis of amebiasis. Am. J. Trop. Med. Hyg., 23 :27–30.
-
7.
Hartmann D. P., Ghadirian E., Meerovitch E., 1980. Enzyme-linked immunosorbent assay and indirect hemagglutination test in serodiagnosis of experimental hepatic amebiasis. J. Parasitol., 66 :344–345.
-
8.
Michel P., Pages J., Albert J. P., Capdevielle P., 1984. Diagnostic de l'amibiase extraintestinale par une méthode ELISA indirecte. Bull. Soc. Pathol. Exot. ,, 77 :234–238.
-
9.
Niel G., Gentilini M., Pinon J.M., Couture J., Danis M., 1974. Electro-synérèse et diagnostic sérologique de l'amibiase: Valeur comparée à celle de l'immuno-electrophorèse, de l'immunofluorescence et du test au latex. Méd. Mal. Infect., 4 :231–235.
-
10.
Pinon J. M., Thoannes H., Gruson N., 1985. An enzyme-linked immuno-filtration assay used to compare infant and maternal antibody profiles in toxoplasmosis. J. Immunol. Meth., 77 :15–23.
-
11.
Pinon J. M., Dropsy G., 1977. ELIEDA (enzyme-linked immuno electro-diffusion-assay): Application of a combined immunoelectrodiffusion and immunoenzyme method to the study of immune response in parasitic infections. J. Immunol. Meth., 16 :15–22.
-
12.
Sharma S. D., Mullenax J., Araujo F. G., Erlich H. A., Remington J. S., 1983. Western blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies. J. Immunol., 131 :977–983.
-
13.
Osisanya J. O. S., Warhurst D. C., 1980. Specific anti-amoebic immunoglobulins and the cellulose acetate precipitin test in Entamoeba histolytica infection. Trans. R. Soc. Trop. Med. Hyg., 74 :605–608.
-
14.
Dasgupta A., 1974. Immunoglobulin in health and disease. III. Immunoglobulins in the sera of patients with amoebiasis. Clin. Exp. Immunol., 86 :163–168.
-
15.
Savanat T., Thammapalerd N., Jaroonvesma N., Bunnag D., 1977. Total serum IgE level in patients with amoebic liver abscess and other parasitic infections. S.E. Asian J. Trop. Med. Pub. Hlth., 8 :149–154.
-
16.
Harris W. G., Friedman M. J., Bray R. S., 1978. Serial measurement of total and parasitespecific IgE in an African population infected with Entamoeba histolytica. Trans. R. Soc. Trop. Med. Hyg., 72 :427–430.
-
17.
Ortiz-Ortiz I., Zamacona G., Sepulveda B., Capin N. R., 1975. Cell-mediated immunity in patients with amebic abscess of the liver. Clin. Immunol. Immunopathol, 4 :127–134.
-
18.
Trissl, D., 1982. Immunology of Entamoeba histolytica in human and animal hosts. Rev. Infect. Dis., 44 :1154–1184.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 347 | 64 | 21 |
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Immunological Studies in Amebiasis: Isotypic Characterization of Specific Antibodies by Enzyme-Linked Immunofiltration Assay
Jean-Michel Pinon
Jean-Michel Pinon
Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 45, rue Cognacq Jay et Rue Alexis Carrel, 51092 Reims Cédex, France
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Jean Poirriez
Jean Poirriez
Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 45, rue Cognacq Jay et Rue Alexis Carrel, 51092 Reims Cédex, France
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Gerard Remy
Gerard Remy
*Service de Medécine Interne et des Maladies Infectieuses, Hôpital Robert Debré, Rue Alexis Carrel, 51092 Reims Cédex, France
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Herve Lepan
Herve Lepan
Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 45, rue Cognacq Jay et Rue Alexis Carrel, 51092 Reims Cédex, France
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Fifty sera from 17 cases of invasive amebiasis (15 hepatic localizations, ameboma, 1 diffused colic localization) were studied by enzyme-linked immunofiltration assay (ELIFA). IgM and IgE anti-Entamoeba histolytica were detected in 10 and 15, respectively, of the 17 patients studied. IgM and IgE antibodies were found simultaneously in 10 cases; these 2 isotypes were only recognized twice in the same serum by the same antigenic components. During post-therapy monitoring, in less than 90 days IgE or IgM-Ab regressed completely in half the cases. If they appeared or reappeared after the third month, prognosis was bad. In addition to its value for diagnosis and prognosis, the ELIFA allowed us to detect the functional antigenic components revealed more particularly by some IgG, IgM, IgE, or IgA antibodies.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Krupp I. M., 1970. Antibody response in intestinal and extraintestinal amebiasis. Am. J. Trop. Med. Hyg., 19 :57–62.
-
2.
Knobloch J., Mannweiler E., 1983. Development and persistence of antibodies to Entamoeba histolytica in patients with amebic liver abscess. Analysis of 216 cases. Am. J. Trop. Med. Hyg., 32 :727–732.
-
3.
Knae G. J., Matossian R., Batty I., 1971. Fluorochrome-labeled anti-immunoglobulin fractions used with stabilized antigen preparations for the assessment of parasitic diseases. Ann. N.Y. Acad. Sci., 177 :134–145.
-
4.
Capron A., Vernes A., Niel G., Bouvry M., 1972. Le diagnostic immunologique de l'amibiase. Med. Chir. Dig., 1 :5–13.
-
5.
Gentilini M., Pinon J. M., Niel G., 1972. Immunoelectrodiffusion sur membrane d'acétate de cellulose. Applications en parasitologie a propos des résultats préliminaires sur 600 essais. Nouv. Presse Méd., 1 :1499–1501.
-
6.
Krupp I. M., 1974. Comparison of counterimmunoelectrophoresis with other serologic tests in the diagnosis of amebiasis. Am. J. Trop. Med. Hyg., 23 :27–30.
-
7.
Hartmann D. P., Ghadirian E., Meerovitch E., 1980. Enzyme-linked immunosorbent assay and indirect hemagglutination test in serodiagnosis of experimental hepatic amebiasis. J. Parasitol., 66 :344–345.
-
8.
Michel P., Pages J., Albert J. P., Capdevielle P., 1984. Diagnostic de l'amibiase extraintestinale par une méthode ELISA indirecte. Bull. Soc. Pathol. Exot. ,, 77 :234–238.
-
9.
Niel G., Gentilini M., Pinon J.M., Couture J., Danis M., 1974. Electro-synérèse et diagnostic sérologique de l'amibiase: Valeur comparée à celle de l'immuno-electrophorèse, de l'immunofluorescence et du test au latex. Méd. Mal. Infect., 4 :231–235.
-
10.
Pinon J. M., Thoannes H., Gruson N., 1985. An enzyme-linked immuno-filtration assay used to compare infant and maternal antibody profiles in toxoplasmosis. J. Immunol. Meth., 77 :15–23.
-
11.
Pinon J. M., Dropsy G., 1977. ELIEDA (enzyme-linked immuno electro-diffusion-assay): Application of a combined immunoelectrodiffusion and immunoenzyme method to the study of immune response in parasitic infections. J. Immunol. Meth., 16 :15–22.
-
12.
Sharma S. D., Mullenax J., Araujo F. G., Erlich H. A., Remington J. S., 1983. Western blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies. J. Immunol., 131 :977–983.
-
13.
Osisanya J. O. S., Warhurst D. C., 1980. Specific anti-amoebic immunoglobulins and the cellulose acetate precipitin test in Entamoeba histolytica infection. Trans. R. Soc. Trop. Med. Hyg., 74 :605–608.
-
14.
Dasgupta A., 1974. Immunoglobulin in health and disease. III. Immunoglobulins in the sera of patients with amoebiasis. Clin. Exp. Immunol., 86 :163–168.
-
15.
Savanat T., Thammapalerd N., Jaroonvesma N., Bunnag D., 1977. Total serum IgE level in patients with amoebic liver abscess and other parasitic infections. S.E. Asian J. Trop. Med. Pub. Hlth., 8 :149–154.
-
16.
Harris W. G., Friedman M. J., Bray R. S., 1978. Serial measurement of total and parasitespecific IgE in an African population infected with Entamoeba histolytica. Trans. R. Soc. Trop. Med. Hyg., 72 :427–430.
-
17.
Ortiz-Ortiz I., Zamacona G., Sepulveda B., Capin N. R., 1975. Cell-mediated immunity in patients with amebic abscess of the liver. Clin. Immunol. Immunopathol, 4 :127–134.
-
18.
Trissl, D., 1982. Immunology of Entamoeba histolytica in human and animal hosts. Rev. Infect. Dis., 44 :1154–1184.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 347 | 64 | 21 |
| Full Text Views | 13 | 6 | 0 |
| PDF Downloads | 3 | 3 | 0 |