Author:
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-
1.
Hommel M., Peters W., Ranque J., Quilici M. & Lanotte G. 1978. The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis. Ann. Trop. Med. Parasitol., 72 :213–218
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2.
Ho M., Leeuwenburg J., Mbugua G., Wamachi A. & Voller A. 1982. An enzyme-linked immunosorbent assay (ELISA) for field diagnosis of visceral leishmaniasis. Am. J. Trop. Med. Hyg., 32 :943–946.
-
3.
Pappas M. G., Hajkowski R., Diggs C. L., Diggs C. L. & Hockmeyer W. T. 1985. Disposable nitrocellulose filtration plates simplify the Dot-ELISA for serodiagnosis of visceral leishmaniasis. Trans. R. Soc. Trop. Med. Hyg., 79 :136
-
4.
Anthony R. L., Christensen H. A. & Johnson C. M. 1980. Micro enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of New World leishmaniasis. Am. J. Trop. Med. Hyg., 29 :190–194
-
5.
Pappas M. G., McGreevy P. B., Hajkowski R., Hendricks L. D., Oster C. N. & Hockmeyer W. T. 1983. Evaluation of promastigote and amastigote antigens in the indirect fluorescent antibody test for American cutaneous leishmaniasis. Am. J. Trop. Med. Hyg., 32 :1260–1267.
-
6.
Sacci J. B., Christensen H. A., Vasquez A. & Anthony R. L. 1987. Serodiagnosis of New World leishmaniasis by using a genus-specific antigen in enzyme-linked immunosorbent assays. Diag. Microbial. Infect. Dis., 6 :229–238.
-
7.
Wirth D. & McMahon-Pratt D. 1982. Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions. Proc. Natl. Acad. Sci. USA, 79 :6999–7003.
-
8.
Lynch N. R., Malave C., Ifante R. B., Modlin R. & Convit J. 1986. In situ detection of amastigotes in American cutaneous leishmaniasis using monoclonal antibodies. Trans. R. Soc. Trop. Med. Hyg., 80 :6–9.
-
9.
Anthony R. L., Williams K. M., Sacci J. B. & Rubin D. C. 1985. Subcellular and taxonomic specificity of monoclonal antibodies to New World Leishmania. Am. J. Trap. Med. Hyg., 34 :1085–1094.
-
10.
Kennett R. H. 1980. Cell fusion. Pages 345–359 inJakoby T. W., Pastan H., eds., Methods in Enzymology, Academic Press, New York.
-
11.
Berman J. D., Goad L. J., Beach D. H. & Zoholc G. G. 1986. Effects of keto-conazole on sterol biosynthesis by Leishmania mexicana mexicana in murine macrophage tumor cells. Mol. Biochem. Parasit., 20 :85–92.
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12.
Sells P. G. & Burton M. 1981. Identification of Leishmania amastigotes and their antigens in formalin fixed tissue by immunoperoxidase staining. Trans. R. Soc. Trap. Med. Hyg., 75 :461–468.
-
13.
Livni N., Abramowitz A., Londner M., Okon E. & Morag A. 1983. Immunoperoxidase method of identification of Leishmania in routinely prepared histological sections. Virch. Arch. (Path. Anal), 401 :147–151.
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14.
Walton B. C., Brooks W. H. & Aronja I. 1972. Serodiagnosis of American leishmaniasis by indirect fluorescent antibody test. Am. J. Trap. Med. Hyg., 21 :296–299.
-
15.
Wirth D. F., Rogers W. O., Barker R., Dourado H., Suebsaeng L. & Albuquerque B. 1986. Leishmaniasis and malaria: New tool for epidemiologic analysis. Science, 234 :975–979.
| Past two years | Past Year | Past 30 Days | |
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Rapid Detection of Leishmania Amastigotes in Fluid Aspirates and Biopsies of Human Tissues
Ronald L. Anthony
Ronald L. Anthony
Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Max Grogl
Max Grogl
* Department of Parasitology, Walter Reed Army Institute of Research, Washington, DC 20307-5100
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John B. Sacci
John B. Sacci
Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Ripley W. Ballou
Ripley W. Ballou
† Department of Immunology, Walter Reed Army Institute of Research, Washington, DC 20307-5100
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When using a genus-specific monoclonal antibody (83-J3D2) as the primary reagent in an indirect immunofluorescent antibody assay (IFA), intracellular amastigotes of Leishmania were easily identified in 9 of 9 biopsies and in 11 of 12 needle aspirates taken from human lesions. In contrast, only 5 of the biopsies and 4 ofthe aspirates yielded promastigotes upon culture in vitro. Similarly, all but 2 of the aspirates and one-half of the biopsies were reported as negative for parasites when stained with Wright's and hematoxylin-eosin, respectively. Serum antibody titers, ranging from 1:8 to 1:128, corroborated the results of the amastigote detection assays when histopathology and isolation were negative.
These findings support the practicality of using the genus-specific monoclonal IFA in those field situations where it becomes necessary to differentiate leishmaniasis from other skin infections.
ProCite
RefWorks
Reference Manager
BibTeX
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-
1.
Hommel M., Peters W., Ranque J., Quilici M. & Lanotte G. 1978. The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis. Ann. Trop. Med. Parasitol., 72 :213–218
-
2.
Ho M., Leeuwenburg J., Mbugua G., Wamachi A. & Voller A. 1982. An enzyme-linked immunosorbent assay (ELISA) for field diagnosis of visceral leishmaniasis. Am. J. Trop. Med. Hyg., 32 :943–946.
-
3.
Pappas M. G., Hajkowski R., Diggs C. L., Diggs C. L. & Hockmeyer W. T. 1985. Disposable nitrocellulose filtration plates simplify the Dot-ELISA for serodiagnosis of visceral leishmaniasis. Trans. R. Soc. Trop. Med. Hyg., 79 :136
-
4.
Anthony R. L., Christensen H. A. & Johnson C. M. 1980. Micro enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of New World leishmaniasis. Am. J. Trop. Med. Hyg., 29 :190–194
-
5.
Pappas M. G., McGreevy P. B., Hajkowski R., Hendricks L. D., Oster C. N. & Hockmeyer W. T. 1983. Evaluation of promastigote and amastigote antigens in the indirect fluorescent antibody test for American cutaneous leishmaniasis. Am. J. Trop. Med. Hyg., 32 :1260–1267.
-
6.
Sacci J. B., Christensen H. A., Vasquez A. & Anthony R. L. 1987. Serodiagnosis of New World leishmaniasis by using a genus-specific antigen in enzyme-linked immunosorbent assays. Diag. Microbial. Infect. Dis., 6 :229–238.
-
7.
Wirth D. & McMahon-Pratt D. 1982. Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions. Proc. Natl. Acad. Sci. USA, 79 :6999–7003.
-
8.
Lynch N. R., Malave C., Ifante R. B., Modlin R. & Convit J. 1986. In situ detection of amastigotes in American cutaneous leishmaniasis using monoclonal antibodies. Trans. R. Soc. Trop. Med. Hyg., 80 :6–9.
-
9.
Anthony R. L., Williams K. M., Sacci J. B. & Rubin D. C. 1985. Subcellular and taxonomic specificity of monoclonal antibodies to New World Leishmania. Am. J. Trap. Med. Hyg., 34 :1085–1094.
-
10.
Kennett R. H. 1980. Cell fusion. Pages 345–359 inJakoby T. W., Pastan H., eds., Methods in Enzymology, Academic Press, New York.
-
11.
Berman J. D., Goad L. J., Beach D. H. & Zoholc G. G. 1986. Effects of keto-conazole on sterol biosynthesis by Leishmania mexicana mexicana in murine macrophage tumor cells. Mol. Biochem. Parasit., 20 :85–92.
-
12.
Sells P. G. & Burton M. 1981. Identification of Leishmania amastigotes and their antigens in formalin fixed tissue by immunoperoxidase staining. Trans. R. Soc. Trap. Med. Hyg., 75 :461–468.
-
13.
Livni N., Abramowitz A., Londner M., Okon E. & Morag A. 1983. Immunoperoxidase method of identification of Leishmania in routinely prepared histological sections. Virch. Arch. (Path. Anal), 401 :147–151.
-
14.
Walton B. C., Brooks W. H. & Aronja I. 1972. Serodiagnosis of American leishmaniasis by indirect fluorescent antibody test. Am. J. Trap. Med. Hyg., 21 :296–299.
-
15.
Wirth D. F., Rogers W. O., Barker R., Dourado H., Suebsaeng L. & Albuquerque B. 1986. Leishmaniasis and malaria: New tool for epidemiologic analysis. Science, 234 :975–979.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 261 | 100 | 5 |
| Full Text Views | 6 | 0 | 0 |
| PDF Downloads | 7 | 0 | 0 |