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-
1.
World Health Organization, 1984. The leishmaniases. WHO Tech. Rep. No. 704.
-
2.
Alexander J., Vickerman K., 1975. Fusion of host cell secondary lysosomes with the parasitophorous vacuoles of Leishmania mexicana-infected macrophages. J. Protozool., 22 :502–508.
-
3.
Ramasamy R., Jamnadas H., Mutinga M. J., 1981. Proteins and surface proteins of Leishmania promastigotes and their possible relevance to the characterization of strains. Intl. J. Parasitol., 11 :387–390.
-
4.
Handman E., Mitchell G. F., Goding J., 1981. Identification and characterization of protein antigens of Leishmania tropica isolates. J. Immunol., 126: 508–512.
-
5.
Chang K. P., Fong D., 1982. Antigenic changes during intracellular differentiation of protein antigens of Leishmania mexicana in cultured macrophages. Infect. Immun., 36 :430–43.
-
6.
Lepay D. A., Nogueira N., Cohn Z., 1983. Surface antigens of Leishmania donovani promastigotes. J. Exp. Med., 157 :1562–1574.
-
7.
Handman E., Hocking R.E., 1982. Stagespecific, strain-specific and cross-reactive antigens of Leishmania species identified by monoclonal antibodies. Infect. Immun., 37 :28–37.
-
8.
Gardiner P. R., Dwyer D. M., 1983. Radiodination and identification of externally disposed membrane components of Leishmania tropica. Mol. Biochem. Parasitol., 8 :283–295.
-
9.
Ramasamy R., Kar S. K, Jamnadas H., 1983. Cross-reacting surface antigens on Leishmania promastigotes. Intl. J. Parasitol., 13 :337–341.
-
10.
Gardiner P. R., Jaffe C. L., Dwyer D. M., 1984. Identification of cross-reactive promastigote cell surface antigens of some leishmanial stocks by 1251 labeling and immunoprecipitation. Infect. Immun., 43 :637–643.
-
11.
Nery-Guimaraes F., Lage H. A., Venancio I. A., Grynberg N. F., 1969. Estudo comparativo da reação indireta de anticorpos fluorescentes em doença de Chagas, leishmanioses tegumentares e calasar com varios antigenos de Leishmania e Trypanosoma. O Hospital, 775 :299–313.
-
12.
Anthony R. L., Christesen H. A., Johnson C. M., 1980. Microenzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of New World leishmaniasis. Am. J. Trop. Med. Hyg., 29 :190–194.
-
13.
Guimarães M. C. S., Celeste B. J., Castilho E. A., Mineo J. R., Diniz J. M. P., 1981. Immunoenzymatic assay (ELISA) in mucocutaneous leishmaniasis, Kala-azar and Chagas#x0027; disease: An epimastigote Trypanosoma cruzi antigen able to distinguish between anti-Trypanosoma and anti-Leishmania antibodies. Am. J. Trap. Med. Hyg., 30 :942–947.
-
14.
Brener Z., Pellegrino J., 1958. Reaoções imunologicas cruzadas em caes com doença de chagas e leishmaniose visceral, natural mente infectados. Rev. Bras. Malarial., 10 :45–49.
-
15.
Momen H., Grimaldi G., Pacheco R. S., Jaffe C. L., McMahon-Pratt D., Marzochi M. C. A., 1985. Brazilian Leishmania stocks phenotypically similar to Leishmania major. Am. J. Trop. Med. Hyg., 34 :1076–1084.
-
16.
Brener Z., Chiari E., 1963. Variaoçães morfologicas observadas em diferentes amostras de Trypanosoma cruzi. Rev. Inst. Med. Trop. São Paulo, 5 :220–224.
-
17.
Silva L. H. P., Nussenzweig V., 1953. Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco. Folia Clin. Biol., 20 :191–208.
-
18.
Camargo E. P., 1964. Growth and differentiation in Trypanosoma cruzi. I. Origin of metacyclic trypanosomes in liquid media. Rev. Inst. Med. Trap. Sao Paulo, 6 :93–100.
-
19.
Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J., 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193 :265–275.
-
20.
Souza W. J. S., Coutinho S. E., Marzochi M. C. A., Toledo L. M., Gottlieb M. V., 1982. Utilização da reaoçãao de imunofluorescencia indireta no acompanhamento da terapeuuca da leishmaniose tegumentar americana. Mem. Inst. O. Cruz, 77 :247–254.
-
21.
Laemmli U. K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 :680–685.
-
22.
Studier F. W., 1973. Analysis of bacteriophage T7 early RNAs and proteins on slab gel. J. Mol. Bioi., 79 :237–248.
-
23.
Towbin H., Staehelin T., Bordon J., 1979. Transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc. Natl. Acad. Sci., USA, 76 :4350–4354.
-
24.
Batteiger B., Newhall W. J. V., Jones R. B., 1982. The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J. Immunol. Meth., 55 :297–307.
-
25.
Ghose A. C., Haldar J. P., Pal S. C., Mishra B. P., Mishra K. K., 1980. Serological investigations on Indian Kala-azar. Clin. Exp. Immunol., 40 :318–326.
-
26.
Behforouz, N. C., Amirhakimi G. H., Rezai H. R., Saberi M. S., 1983. Immunological findings in Kala-azar, Iran. Trap. Geogr. Med., 35 :27–32.
-
27.
Galvão-Castro B., Sá-Ferreira J. A., Marzochi K. F., Marzochi M. C., Coutinho S. G., Lambert P. H., 1984. Polyclonal B-cell activation circulating immune complexes and autoimmunity in human visceral leishmaniasis. Clin. Exp. Immunol., 56 :727–733.
-
28.
Manson-Bahr P. E. C., 1961. Immunity in Kala-azar. Trans. R. Soc. Trop. Med. Hyg., 55 :550–555.
-
29.
Shaw J. S., Voller A., 1964. The detection of circulating antibody to Kala-azar by means of immunofluorescent techniques.. Trans. R. Soc. Trop. Med. Hyg., 58 :349–352.
-
30.
Afchain D., Le Ray D., Fruit J., Capron A., 1979. Antigenic make-up of Trypanosoma cruzi culture forms: Identification of a specific component. J. Parasitol., 65 :507–514.
-
31.
Colomer-Gold V., Quintão, L. G., Keithly J., Nogueira N., 1985. A common major surface antigen on amastigotes and promastigotes of Leishmania species. J. Exp. Med., 162 :902–916.
-
32.
Pearson R. D., Steinbrigel R. T., 1980. Mechanism of lethal effect of human serum upon Leishmania donovani. J. Immunol., 125 :2195–2201.
-
33.
Smrkovski L. L., Larson L., 1977. Antigenic cross-reactivity between Mycobacterium bovis (BCG) and Leishmania donovani. Infect. Immun., 18 :561–562.
-
34.
Decker-Jackson J. E., Honigberg B. M., 1978. Glycoproteins released by Leishmania donovani: Immunologic relationships with host and bacterial antigens and preliminary biochemical analysis. J. Protozool., 25 :514–525.
-
35.
Torrealba J. W., Chaves-Torrealba J., 1964. BCG antigen used in the complement-fixation test for the diagnosis of Kala-azar. Rev. Inst. Med. Trap. São Paulo, 6 :252–253.
-
36.
Alencar J. E. de, Ilardi A., Pampiglione, S., 1968. La reazione di fissazione del complemento con antigene da B.C. G. nella leishmaniosi viscerale. Parassitologia, 10 :33–35.
-
37.
Bray R. S., Laison R., 1966. Immunology and serology of leishmaniasis. IV. Results of ouchterlony double diffusion tests. Trans. R. Soc. Trop. Med. Hyg., 60 :605–609.
-
38.
Bray R. S., Laison R., 1967. Studies on the immunology and serology of leishmaniasis. V. The use of particles as vehicles in passive agglutination tests. Trans. R. Soc. Trop. Med. Hyg., 61 :490–505.
-
39.
Kohanteb J., Ardehali S., Rezai H. R., 1980. Studies on antigenic relationship of Leishmania promastigotes by electroimmunodiffusion and crossed electroimmunodiffusion tests. Trans. R. Soc. Trop. Med. Hyg., 74 :582–584.
-
40.
McMahon-Pratt D., David J. R., 1981. Monoclonal antibodies that distinguish between New World species of Leishmania. Nature, 291 :581–583.
-
41.
McMahon-Pratt D., David J. R., 1982. Monoclonal antibodies recognizing determinants specific for the promastigote stage of Leishmania mexicana. Mol. Biochem. Parasitol., 6 :317–327.
-
42.
Jaffe C. L., McMahon-Pratt D., 1983. Monoclonal antibodies specific for Leishmania tropica: I. Characterization of antigens associated with stage and species-specific determinants. J. Immunol., 131 :1987–1993.
-
43.
Jaffe C. L., Bennet E., Bennet G., McMahon-Pratt D., 1984. Production and characterization of species-specific monoclonal antibodies against Leishmania donovani for immunodiagnosis. J. Immunol.,, 133 :440–447.
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Human Visceral Leishmaniasis: Analysis of the Specificity of Humoral Immune Response to Polypeptides of Leishmania Donovani Chagasi
Soluble antigens from Leishmania donovani chagasi were studied in terms of their ability to react with sera from human visceral leishmaniasis. Thirty-six polypeptides, with molecular weights ranging from 14,400 to 123,000 were demonstrated by Western blot analysis. An extensive cross-reactivity with sera from patients with cutaneous leishmaniasis and Chagas' disease also was observed. Two polypeptides (Mr 119,000 and 123,000) reacted with all the sera from visceral leishmaniasis patients. When they were electroeluted from gels and evaluated with respect to specificity to the L. donovani chagasi subspecies, these components were expressed in all strains of Leishmania tested, but not in those of Trypanosoma cruzi. These results indicated that these components are shared by Trypanosomatidae of genus Leishmania. The eluted polypeptides did not react with sera from patients with Chagas' disease, indicating the feasibility of using purified antigens to discriminate between the humoral immune responses in T. cruzi and Leishmania infections.