Natural Infection of Humans, Animals, and Phlebotomine Sand Flies with the Alagoas Serotype of Vesicular Stomatitis Virus in Colombia

Robert B. TeshYale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510

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S. Jorge BoshellInstituto Nacional de Salud, Ministerio de Salud, Bogotá, Colombia

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Govind B. ModiYale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510

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A. Alberto MoralesInstituto Nacional de Salud, Ministerio de Salud, Bogotá, Colombia

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David G. YoungDepartment of Entomology and Nematology, University of Florida, Gainesville, Florida 32601

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A. Augusto CorredorInstituto Nacional de Salud, Ministerio de Salud, Bogotá, Colombia

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Cristina Ferro de CarrasquillaInstituto Nacional de Salud, Ministerio de Salud, Bogotá, Colombia

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Cristina de RodriguezInstituto Nacional de Salud, Ministerio de Salud, Bogotá, Colombia

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Laurel L. WaltersYale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510

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Marta O. GaitanInstituto Nacional de Salud, Ministerio de Salud, Bogotá, Colombia

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Five isolations of the Alagoas serotype of vesicular stomatitis virus (Rhabdoviridae: Vesiculovirus) were made from naturally infected phlebotomine sand flies (Lutzomyia spp.) collected in Colombia. These are the first isolations of Alagoas virus from an arthropod. Replication of the virus occurred in laboratory-reared sand flies (Lutzomyia longipalpis) after inoculation. Bite and transovarial transmission of the virus was also demonstrated in experimentally infected sand flies. Alagoas virus neutralizing antibodies were found in sera of humans and animals living near the insect collection site; antibody rates among human residents of two nearby towns were 63% and 83%, respectively. Results of comparative serologic studies demonstrated that Alagoas virus is closely related antigenically to Indiana, Cocal, and Maraba viruses and that these four agents form a complex within the vesicular stomatitis virus serogroup. The antigenic similarity among these four viruses makes their differentiation difficult; it also raises doubts about the accuracy of current laboratory methods used for identifying isolates in this serogroup. A discussion follows on the significance of human antibodies to these agents and on the role of sand flies in their ecology.

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