Author:
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1672 | 1404 | 259 |
| Full Text Views | 8 | 3 | 1 |
| PDF Downloads | 4 | 3 | 0 |
Effect of Murine Polymorphonuclear Leukocytes on the Yeast Form of Paracoccidioides Braziliensis
Juan G. McEwen
Juan G. McEwen
Laboratorio de Micologia, Corporacion para Investigaciones Biologicas, AA #73-78, Medellin, Colombia
Search for other papers by Juan G. McEwen in
Current site
Google Scholar
PubMed
Search for other papers by Juan G. McEwen in
Current site
Google Scholar
PubMed
Elmer Brummer
Elmer Brummer
Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center and Institute for Medical Research, Stanford University Medical School, San Jose, California 95128
Search for other papers by Elmer Brummer in
Current site
Google Scholar
PubMed
Search for other papers by Elmer Brummer in
Current site
Google Scholar
PubMed
David A. Stevens
David A. Stevens
Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center and Institute for Medical Research, Stanford University Medical School, San Jose, California 95128
Search for other papers by David A. Stevens in
Current site
Google Scholar
PubMed
Search for other papers by David A. Stevens in
Current site
Google Scholar
PubMed
Angela Restrepo
Angela Restrepo
Laboratorio de Micologia, Corporacion para Investigaciones Biologicas, AA #73-78, Medellin, Colombia
Search for other papers by Angela Restrepo in
Current site
Google Scholar
PubMed
Search for other papers by Angela Restrepo in
Current site
Google Scholar
PubMed
The fungicidal activity of murine polymorphonuclear neutrophils from the peripheral blood or elicited intraperitoneally with thioglycollate or with antigen in Paracoccidioides braziliensis-sensitized or nonsensitized mice was studied. Although peripheral blood, thioglycollate-elicited, and antigen-elicited neutrophils from normal mice or thiogly-collate-elicited neutrophils from P. braziliensis-sensitized mice killed Candida albicans (57% to 84%), they failed to significantly reduce inoculum colony forming units of P. braziliensis (0% to 13%). In contrast, antigen-elicited neutrophils from sensitized mice reduced colony forming units of P. braziliensis by 40%, and exhibited significantly enhanced candidacidal activity compared to thioglycollate-elicited neutrophils from normal or sensitized mice but not peripheral blood neutrophils from normal mice. Fresh serum, but not specific antibody, was required for optimal killing of P. braziliensis, presumably representing an essential role for complement. Killing of P. braziliensis by antigen-elicited neutrophils from sensitized mice correlated with their ability to produce an enhanced oxidative burst, as measured by luminol-assisted chemiluminescence, when interacting with killed P. braziliensis cells. These results indicate that in P. braziliensis-sensitized hosts an inflammatory reaction to P. braziliensis results in activation of neutrophils for significant killing of the pathogen.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1672 | 1404 | 259 |
| Full Text Views | 8 | 3 | 1 |
| PDF Downloads | 4 | 3 | 0 |