Monoclonal Antibodies against Dengue 2 Virus E-Glycoprotein Protect Mice against Lethal Dengue Infection

B. M. Kaufman Department of Biologics Research, Walter Reed Army Institute of Research, Washington, DC 20307

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P. L. Summers Department of Biologics Research, Walter Reed Army Institute of Research, Washington, DC 20307

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D. R. Dubois Department of Biologics Research, Walter Reed Army Institute of Research, Washington, DC 20307

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K. H. Eckels Department of Biologics Research, Walter Reed Army Institute of Research, Washington, DC 20307

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DOI:
https://doi.org/10.4269/ajtmh.1987.36.427
Volume/Issue:
Volume 36: Issue 2
Page(s):
427–434
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A panel of 11 murine monoclonal antibodies directed against dengue type 2 was evaluated for antigen specificity by dot immunobinding assay and Western blot analysis and for in vitro and in vivo biological activities. Nine of the 11 monoclonal antibodies reacted with viral E-glycoprotein based on the Western blot analysis; one reacted with a 36 Kd protein present in dengue-infected C6/36 mosquito cells. The nine E-glycoprotein-reactive monoclonal antibodies also neutralized dengue 2 virus in a plaque reduction assay. Of the neutralizing monoclonal antibodies, five passively protected mice in vivo against lethal intracerebral dengue 2 challenge. The protective monoclonal antibodies were directed against viral determinants that fell into at least three spatially separate families of epitopes on E-glycoprotein, the antigenicities of which were preserved after heat/detergent denaturation.
Copyright:
Copyright © 1987 by The American Society of Tropical Medicine and Hygiene 1987
Accepted:
14 Oct 1986
|
Published Online:
Mar 1987
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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