Biology of Arboledas Virus, A New Phlebotomus Fever Serogroup Virus (Bunyaviridae: Phlebovirus) Isolated from Sand Flies in Colombia

Robert B. TeshYale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, P.O. Box 3333, New Haven, Connecticut 06510

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S. Jorge BoshellInstituto Nacional de Salud, Ministerio de Salud, Apartado Aereo 80080, Bogotá, Colombia

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David G. YoungDepartment of Entomology and Nematology, University of Florida, Gainesville, Florida 32611

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A. Alberto MoralesInstituto Nacional de Salud, Ministerio de Salud, Apartado Aereo 80080, Bogotá, Colombia

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A. Agusto CorredorInstituto Nacional de Salud, Ministerio de Salud, Apartado Aereo 80080, Bogotá, Colombia

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Govind B. ModiYale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, P.O. Box 3333, New Haven, Connecticut 06510

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Cristina Ferro De CarrasquillaInstituto Nacional de Salud, Ministerio de Salud, Apartado Aereo 80080, Bogotá, Colombia

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Cristina De RodriguezInstituto Nacional de Salud, Ministerio de Salud, Apartado Aereo 80080, Bogotá, Colombia

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Marta O. GaitanInstituto Nacional de Salud, Ministerio de Salud, Apartado Aereo 80080, Bogotá, Colombia

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Six isolates of a new phlebotomus fever serogroup virus, designated Arboledas virus, were obtained from sand flies (Lutzomyia spp.) collected in northeastern Colombia. One of the isolates was made from a pool of male sand flies. By immunofluorescence, Arboledas virus is related to Caimito and Pacui viruses; by neutralization test, it is distinct. Arboledas virus neutralizing antibodies were found in the sera of opossums (Didelphis marsupialis) and humans living in the study area. D. marsupialis inoculated with the virus developed a viremia of four days' duration, and sand flies (Lutzomyia gomezi) feeding on a viremic opossum were readily infected. Transovarial transmission of Arboledas virus was also demonstrated in experimentally infected Lu. gomezi. Results of the above laboratory studies suggest that Arboledas virus is maintained in nature by two mechanisms: vertical (transovarial) transmission in the insect vector, and an alternating marsupial-sand fly cycle. The implications of this complex maintenance cycle for other phleboviruses are discussed.

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