Leishmania donovani: Immunization against Infection as a Function of Parasite Growth Phase

J. C. Jarecki-Black Departments of Laboratory Medicine and Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425

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E. R. James Departments of Laboratory Medicine and Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425

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J. W. Kirshtein Departments of Laboratory Medicine and Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425

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J. D. Kirshtein Departments of Laboratory Medicine and Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425

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A. B. Glassman Departments of Laboratory Medicine and Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425

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DOI:
https://doi.org/10.4269/ajtmh.1986.35.1117
Volume/Issue:
Volume 35: Issue 6
Page(s):
1117–1120
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C57BL/6 mice were immunized against Leishmania donovani infection with a subcutaneous vaccination protocol. Groups received 3 injections at 4-day intervals combining glucan and killed promastigotes harvested from either logarithmic or stationary phase cultures. Controls were immunized with glucan alone, stationary or log phase promastigotes alone, or were untreated. All groups were challenged intravenously with stationary phase promastigotes at day 45 post-immunization. Results revealed that animals immunized with the glucan-killed parasite vaccine, utilizing promastigotes derived from either log (GPL) or stationary phase cultures (GPs), demonstrated significant resistance against infection as compared to controls or untreated mice. Additionally, the reduction in hepatic amastigote proliferation in mice immunized with GPs was significantly greater than in mice immunized with GPL.

Author Notes

Copyright:
Copyright © 1986 by The American Society of Tropical Medicine and Hygiene 1986
Accepted:
12 Jun 1986
|
Published Online:
Nov 1986
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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