Evaluation of a Synthetic Oligonucleotide Probe for Diagnosis of Plasmodium falciparum Infections

Cathleen M. Mucenski

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Patricia Guerry Infectious Disease Program Center, U.S. Naval Medical Research Institute, Bethesda, Maryland

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Mary Buesing

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Ana Szarfman

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James Trosper Infectious Disease Program Center, U.S. Naval Medical Research Institute, Bethesda, Maryland

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David Walliker National Institutes of Health, Bethesda, Maryland

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George Watt U.S. Naval Medical Research Unit No. 2, Manila, Philippines

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Ruperto Sangalang San Lazaro Hospital, Manila, Philippines

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Catherine P. Ranoa San Lazaro Hospital, Manila, Philippines

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Melinda Tuazon U.S. Naval Medical Research Unit No. 2, Manila, Philippines

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Olegario R. Majam Infectious Disease Program Center, U.S. Naval Medical Research Institute, Bethesda, Maryland

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Isabella Quakyi National Institutes of Health, Bethesda, Maryland

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Leonard W. Scheibel Uniformed Services University of the Health Sciences, Bethesda, Maryland

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John H. Cross Uniformed Services University of the Health Sciences, Bethesda, Maryland

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Peter L. Perine Uniformed Services University of the Health Sciences, Bethesda, Maryland

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DOI:
https://doi.org/10.4269/ajtmh.1986.35.912
Volume/Issue:
Volume 35: Issue 5
Page(s):
912–920
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A radiolabeled synthetic oligonucleotide was evaluated as a diagnostic probe specific for Plasmodium falciparum using blood samples lysed directly on nitrocellulose filters. The probe technique successfully diagnosed malaria in experimentally infected chimpanzees that had 0.001% parasitemias (50 parasites/µl) as determined by blood smears, and in 1 chimpanzee whose blood smear was negative, but whose blood was culture-positive for P. falciparum. In a double blind study of 50 patient samples from the Philippines, the probe results correlated well with blood smear results when the autoradiographs were read after 4–8 hr exposure. The results indicate that the oligonucleotide probe may be useful in the rapid and specific diagnosis of P. falciparum infection.
Copyright:
Copyright © 1986 by The American Society of Tropical Medicine and Hygiene 1986
Accepted:
02 May 1986
|
Published Online:
Sep 1986
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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