Identification of African Swine Fever Viral Antigens in the Hemolymph of Soft Ticks (Argasidae: Ornithodoros) by the Immunodot Blot Test

G. GeeringU.S. Department of Agriculture, Agricultural Research Service, North Atlantic Area, Plum Island Animal Disease Center, P.O. Box 848, Greenport, New York 11944

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R. G. EndrisU.S. Department of Agriculture, Agricultural Research Service, North Atlantic Area, Plum Island Animal Disease Center, P.O. Box 848, Greenport, New York 11944

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T. M. HaslettU.S. Department of Agriculture, Agricultural Research Service, North Atlantic Area, Plum Island Animal Disease Center, P.O. Box 848, Greenport, New York 11944

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P. L. DoroskiU.S. Department of Agriculture, Agricultural Research Service, North Atlantic Area, Plum Island Animal Disease Center, P.O. Box 848, Greenport, New York 11944

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W. R. HessU.S. Department of Agriculture, Agricultural Research Service, North Atlantic Area, Plum Island Animal Disease Center, P.O. Box 848, Greenport, New York 11944

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The immunodot blot test was used to identify African swine fever virus (ASFV) antigens in the hemolymph from soft ticks (Ornithodoros coriaceus) fed on ASFV-infected pigs. The immunodot blot test was sensitive and specific for ASFV antigens and has potential field application. Hemolymph from field-collected ticks can be screened for ASFV and a variety of other tick-borne pathogens using this test.

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