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Identification of Brugia Malayi in Vectors with a Species-Specific DNA Probe
Betty Kim Lee Sim
Betty Kim Lee Sim
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts
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Joon Wah Mak
Joon Wah Mak
Divisions of Filariasis and Malaria and Entomology, Institute for Medical Research, Kuala Lumpur, Malaysia
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Weng Hui Cheong
Weng Hui Cheong
Divisions of Filariasis and Malaria and Entomology, Institute for Medical Research, Kuala Lumpur, Malaysia
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Inge Sutanto
Inge Sutanto
Department of Parasitology, University of Indonesia, Jakarta, Indonesia
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Liliana Kurniawan
Liliana Kurniawan
National Institute of Health, Research and Development of the Ministry of Health, Jakarta, Indonesia
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Haryani A. Marwoto
Haryani A. Marwoto
National Institute of Health, Research and Development of the Ministry of Health, Jakarta, Indonesia
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Eileen Franke
Eileen Franke
United States Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia
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James R. Campell
James R. Campell
United States Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia
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Dyann F. Wirth
Dyann F. Wirth
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts
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Willy F. Piessens
Willy F. Piessens
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts
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We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm 15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, Breinlia booliati or Cardiofilaria species, animal filariids that can be sympatric with B. malayi. P32-labeled clone pBm 15 correctly identified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.
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| Past two years | Past Year | Past 30 Days | |
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