A Novel Lipid-Conjugated Marker for the Direct Labeling of the Schistosomulum Membrane

Francesca Levi-Schaffer Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

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Rebeca Tarrab-Hazdai Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

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Ruth Arnon Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

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Moshe Smolarsky Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

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DOI:
https://doi.org/10.4269/ajtmh.1986.35.544
Volume/Issue:
Volume 35: Issue 3
Page(s):
544–548
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Introduction of the fluorescent group lissamine rhodamine B into the surface of schistosomula of S. mansoni was achieved by brief incubation of the worms with liposomes carrying the lipid bound fluorophore in their bilayers. The liposomers were made of egg lecithin (PC) and lissamine-phosphatidylethanolamine (lissamine-PE). The lissamine groups could be directly detected on the parasite membrane by observation of the single worms under a fluorescent microscope. The fluorescent marker was employed to measure the lateral diffusion coefficient (D) of lipids in the schistosomular membrane and as a surface marker during the isolation of the schistosomula surface membrane.
Copyright:
Copyright © 1986 by The American Society of Tropical Medicine and Hygiene 1986
Accepted:
16 Dec 1985
|
Published Online:
May 1986
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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