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A Novel Lipid-Conjugated Marker for the Direct Labeling of the Schistosomulum Membrane
Francesca Levi-Schaffer
Francesca Levi-Schaffer
Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Rebeca Tarrab-Hazdai
Rebeca Tarrab-Hazdai
Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Ruth Arnon
Ruth Arnon
Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Moshe Smolarsky
Moshe Smolarsky
Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Introduction of the fluorescent group lissamine rhodamine B into the surface of schistosomula of S. mansoni was achieved by brief incubation of the worms with liposomes carrying the lipid bound fluorophore in their bilayers. The liposomers were made of egg lecithin (PC) and lissamine-phosphatidylethanolamine (lissamine-PE). The lissamine groups could be directly detected on the parasite membrane by observation of the single worms under a fluorescent microscope. The fluorescent marker was employed to measure the lateral diffusion coefficient (D) of lipids in the schistosomular membrane and as a surface marker during the isolation of the schistosomula surface membrane.
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