Use of Modified Grace's Insect Medium for the Primary Isolation of Leishmania Donovani Donovani in Bihar, India

R. Saran Divisions of

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A. K. Gupta Divisions of

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S. N. Shrivastava Parasitology

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L. S. N. Prasad Parasitology

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DOI:
https://doi.org/10.4269/ajtmh.1986.35.488
Volume/Issue:
Volume 35: Issue 3
Page(s):
488–490
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Grace's insect medium, supplemented with 20% (v/v) heat-inactivated fetal bovine serum (FBS) and 15% defibrinated pooled rabbit blood is statistically shown to be more sensitive than modified NNN medium (P < 0.05), Grace's insect medium with 20% (v/v) and Grace's insect medium with 30% (v/v) FBS (P <0.01) for in vitro primary isolation of promastigotes of Leishmania donovani donovani from cultures of bone marrow aspirates. This medium has been found to be especially useful for parasitological diagnosis of untreated cases of visceral leishmaniasis and for cases where microscopic examination of bone marrow aspirates has been negative for Leishman-Donovan bodies.

Author Notes

Copyright:
Copyright © 1986 by The American Society of Tropical Medicine and Hygiene 1986
Accepted:
02 Jan 1986
|
Published Online:
May 1986
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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