Anti-Plasmodium falciparum Antibodies Acquired by Residents in a Holoendemic Area of Liberia During Development of Clinical Immunity

Mats Wahlgren

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Anders Björkman

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Hedvig Perlmann Department of Immunology, University of Stockholm, S-106 91 Stockholm, Sweden

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Klavs Berzins Department of Immunology, University of Stockholm, S-106 91 Stockholm, Sweden

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Peter Perlmann Department of Immunology, University of Stockholm, S-106 91 Stockholm, Sweden

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DOI:
https://doi.org/10.4269/ajtmh.1986.35.22
Volume/Issue:
Volume 35: Issue 1
Page(s):
22–29
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Sera from 48 children and adolescents (2–15 years of age), residing in a malaria holoendemic area of Liberia were investigated for specificities and isotypes of anti-P. falciparum antibodies. No clear-cut relationship to the development of clinical immunity was found when the overall antibody activities to total parasite antigens were determined by enzyme-linked immunosorbent assay (ELISA). Although there was a certain rise of IgM, total IgG- and IgG2 antibody activities, this was most pronounced at ages when a clinical but nonsterile immunity is already present. When the sera were investigated by immunoprecipitation of 35S-methionine labeled parasite polypeptides, the total number of parasite antigens precipitated was similar at all ages. Analysis by indirect immunofluorescence (IFA), registering antibodies to intracellular parasite antigens, revealed no age-dependent changes in antibody titers. In contrast, when the sera were assayed by a novel IFA, specific for a restricted number of parasite antigens in the membrane of infected erythrocytes, the frequency of positive sera as well as the anti-P. falciparum titers rose in parallel with the development of clinical immunity. Thus, these antigens appeared to be important inducers of protective immune responses and may be suitable candidates for a vaccine against the asexual blood stages of P. falciparum.
Accepted:
03 Jul 1985
|
Published Online:
Jan 1986
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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