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Isolation and Characterization of Hemorrhagic Toxin g from the Venom of Crotalus Atrox (Western Diamondback Rattlesnake)
Toshiaki Nikai
Toshiaki Nikai
Faculty of Pharmacy, Meijo University, Tenpaku-Ku, Nagoya, Japan
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Nobuhiro Mori
Nobuhiro Mori
Faculty of Pharmacy, Meijo University, Tenpaku-Ku, Nagoya, Japan
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Mitsuhiro Kishida
Mitsuhiro Kishida
Faculty of Pharmacy, Meijo University, Tenpaku-Ku, Nagoya, Japan
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Makoto Tsuboi
Makoto Tsuboi
Faculty of Pharmacy, Meijo University, Tenpaku-Ku, Nagoya, Japan
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Hisayoshi Sugihara
Hisayoshi Sugihara
Faculty of Pharmacy, Meijo University, Tenpaku-Ku, Nagoya, Japan
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Hemorrhagic toxin g (HT-g) was isolated from Crotalus atrox (western diamondback rattlesnake) venom using a five-step purification procedure to obtain ā5.9 mg of purified HT-g from 2.0 g of crude venom. The purified toxin was homogeneous by disc electrophoresis on polyacrylamide gel at pH 8.3 and 4.3, and by isoelectric focusing. HT-g possessed lethal, hemorrhagic and proteolytic activities. These activities of toxin were inhibited by ethylenediamine-tetraacetic acid (EDTA), 1,10-phenanthroline or ethyleneglycol (Ī²-amino-ethyl) N,N,Nā²,Nā²-tetracetic acid (EGTA), but not by cysteine or soybean trypsin inhibitor (SBTI). Its molecular weight was approximately 60,000 and the isoelectric point was 6.8. The toxin contains 516 amino acid residues. HT-g did not coagulate fibrinogen to fibrin; however, the toxin hydrolysed the AĪ±-chain or BĪ²-chain of fibrinogen without cleaving the Ī³-chain. HT-g produced only local hemorrhage in internal organs such as the intestine, heart and liver.
| Past two years | Past Year | Past 30 Days | |
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| PDF Downloads | 6 | 0 | 0 |