Cloning and Characterization of Mefloquine-Resistant Plasmodium Falciparum from Thailand

H. Kyle Webster
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S. Thaithong Chulalongkorn University, Bangkok, Thailand

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K. Pavanand
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K. Yongvanitchit
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C. Pinswasdi Chulalongkorn University, Bangkok, Thailand

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E. F. Boudreau
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DOI:
https://doi.org/10.4269/ajtmh.1985.34.1022
Volume/Issue:
Volume 34: Issue 6
Page(s):
1022–1027
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Resistance to mefloquine in Plasmodium falciparum has begun to occur along the border of Thailand and Kampuchea. As a means of assessing the natural occurrence of mefloquine resistance, the admission and post-treatment parasite isolates from a mefloquine treatment failure were cloned and characterized. Clones from the admission isolate were susceptible to mefloquine in vitro (ID50 of 3.4 [2–5], G [95% CI] ng/ml) and showed a mixture of isozyme types for glucose phosphate isomerase (GPI types I and II). The post-treatment clones were resistant to mefloquine in vitro (ID50 of 17.3 [13–23] ng/ml) with only one isozyme (GPI type I) detected. These observations suggest that under mefloquine pressure a resistant parasite population was selected in the patient, indicating that the potential for mefloquine resistance already exists in the indigenous P. falciparum gene pool. In addition, the mefloquine-resistant clones showed decreased susceptibility in vitro to halofantrine suggesting possible cross-resistance to this new antimalarial drug currently under development.
Copyright:
Copyright © 1985 by The American Society of Tropical Medicine and Hygiene 1985
Accepted:
24 Jun 1985
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Published Online:
Nov 1985
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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