A Field Method for the Procurement of Infective Larvae of Onchocerca Volvulus

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  • Division of Communicable Diseases, Office of Health, Bureau for Science and Technology, Agency for International Development, Department of Immunology and Infectious Diseases, The Johns Hopkins School of Hygiene and Public Health, Washington, DC

Natural vectors of onchocerciasis (S. yahense) were collected. Ninety-eight were intrathoracically inoculated with 30 freshly obtained skin-dwelling microfilariae. Of the 82 flies dissected, 56 were infected and 28 harbored infective larvae. A total of 193 infective O. volvulus larvae were obtained. The mean infective worm burden was 6.9.