Detection of Plasmodium Falciparum in Blood Using DNA Hybridization

Yaakov Pollack Department of Cellular Biochemistry

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Shulamit Metzger Department of Parasitology, The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel

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Ruth Shemer Department of Cellular Biochemistry

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Daniel Landau Department of Cellular Biochemistry

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Dan T. Spira Department of Parasitology, The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel

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Jacob Golenser Department of Parasitology, The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel

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DOI:
https://doi.org/10.4269/ajtmh.1985.34.663
Volume/Issue:
Volume 34: Issue 4
Page(s):
663–667
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A rapid and simple assay for detecting Plasmodium falciparum in human blood was developed. The assay is based on DNA-DNA spot hybridization, using radiolabeled P. falciparum DNA as a probe and finger prick blood as the assay sample. It is very sensitive, able to detect parasitemia levels of 0.0001% in 10 µl of blood. The assay can be quantified and used to estimate parasitemia levels. Several hundred blood samples can be processed simultaneously, and the entire procedure is completed within 24 hr. This assay can be useful for epidemiological surveys, for screening of blood by blood banks and for health authorities examining immigrants and tourists coming from malaria infested areas.

Author Notes

Copyright:
Copyright © 1985 by The American Society of Tropical Medicine and Hygiene 1985
Accepted:
31 Jan 1985
|
Published Online:
Jul 1985
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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