The Development of a Solid Phase Radioimmunoassay for the Detection of Leishmanial Parasites in the Sand Fly

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  • Kuvin Centre and the Department of Parasitology, Hebrew University-Hadassah Medical School, Jerusalem, Israel

The development of a solid phase radioimmunoassay inhibition test using anti-leishmanial monoclonal antibodies is described for the detection of leishmanial promastigote infections in sand flies, as an aid to epidemiology. As few as 6–12 Leishmania major promastigotes could be detected in laboratory-bred Phlebotomus papatasi. The test was sensitive and species-specific.