Antimalarial Drug Susceptibility Testing of Plasmodium Falciparum in Thailand Using a Microdilution Radioisotope Method

H. Kyle Webster U.S. Army Medical Component, AFRIMS, Rajavithi Road, Bangkok, Thailand

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Ellen F. Boudreau U.S. Army Medical Component, AFRIMS, Rajavithi Road, Bangkok, Thailand

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Katchrinnee Pavanand U.S. Army Medical Component, AFRIMS, Rajavithi Road, Bangkok, Thailand

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Kosol Yongvanitchit U.S. Army Medical Component, AFRIMS, Rajavithi Road, Bangkok, Thailand

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Lorrin W. Pang U.S. Army Medical Component, AFRIMS, Rajavithi Road, Bangkok, Thailand

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DOI:
https://doi.org/10.4269/ajtmh.1985.34.228
Volume/Issue:
Volume 34: Issue 2
Page(s):
228–235
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Antimalarial activity of chloroquine, quinine, mefloquine and halofantrine against 33 strains of P. falciparum isolated from naturally acquired malaria infections in Thailand was determined using a radioisotope microdilution method. A microtitration procedure was used to test isolates of P. falciparum against the 4 drugs simultaneously. The mean ID50 for chloroquine and quinine reflected known resistance to those drugs in Thailand. The mean ID50 for mefloquine and halofantrine showed susceptibility to these drugs. Four isolates of P. falciparum however had markedly decreased susceptibility to melfloquine (ID50 > 15 ng/ml); one case of which was confirmed as the first case of RII resistance for mefloquine in Thailand. Several parasite isolates were also observed to have decreased susceptibility to the new drug, halofantrine. These studies strongly recommend that in vitro testing be done in conjunction with field evaluation of new antimalarial drugs.

Author Notes

Copyright:
Copyright © 1985 by The American Society of Tropical Medicine and Hygiene 1985
Accepted:
23 Aug 1984
|
Published Online:
Mar 1985
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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