In Vitro Culture of Two Populations (Dividing and Nondividing) of Exoerythrocytic Parasites of Plasmodium Vivax

Michael R. HollingdaleBiomedical Research Institute, Malaria Branch Centers for Disease Control, Division of Pediatric Hematology Oncology, Dana Farber Cancer Institute, Children's Hospital Medical Center, Department of Pediatrics, Harvard Medical School, 12111 Parklawn Drive, Rockville, Maryland 20852, Georgia

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William E. CollinsBiomedical Research Institute, Malaria Branch Centers for Disease Control, Division of Pediatric Hematology Oncology, Dana Farber Cancer Institute, Children's Hospital Medical Center, Department of Pediatrics, Harvard Medical School, 12111 Parklawn Drive, Rockville, Maryland 20852, Georgia

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Carlos C. CampbellBiomedical Research Institute, Malaria Branch Centers for Disease Control, Division of Pediatric Hematology Oncology, Dana Farber Cancer Institute, Children's Hospital Medical Center, Department of Pediatrics, Harvard Medical School, 12111 Parklawn Drive, Rockville, Maryland 20852, Georgia

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Alan L. SchwartzBiomedical Research Institute, Malaria Branch Centers for Disease Control, Division of Pediatric Hematology Oncology, Dana Farber Cancer Institute, Children's Hospital Medical Center, Department of Pediatrics, Harvard Medical School, 12111 Parklawn Drive, Rockville, Maryland 20852, Georgia

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Plasmodium vivax sporozoites invaded human hepatoma cells and differentiated into 2 types of exoerythrocytic (EE) parasites. One group was composed of actively dividing schizonts, which released merozoites after 9 days of culture. The second group was nondividing and persisted after the primary schizonts disappeared from the culture. EE schizonts progressively lost reactivity to monoclonal antibodies to the surface-protective protein antigen of P. vivax sporozoites, but the persisting parasites remained strongly reactive. In the 2 strains of P. vivax studied, the ratio of schizonts to persisting parasites was approximately equal.

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