| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1110 | 620 | 25 |
| Full Text Views | 4 | 1 | 0 |
| PDF Downloads | 2 | 0 | 0 |
Evaluation of a Purified Schistosoma japonicum Glycoprotein Egg Antigen for the Immunodiagnosis of Infection in Man
J. W. Tracy
J. W. Tracy
Search for other papers by J. W. Tracy in
Current site
Google Scholar
PubMed
Search for other papers by J. W. Tracy in
Current site
Google Scholar
PubMed
E. O. Domingo
E. O. Domingo
Department of Medicine, Philippine General Hospital, Manila, Philippines
Search for other papers by E. O. Domingo in
Current site
Google Scholar
PubMed
Search for other papers by E. O. Domingo in
Current site
Google Scholar
PubMed
A. A. F. Mahmoud
A. A. F. Mahmoud
Search for other papers by A. A. F. Mahmoud in
Current site
Google Scholar
PubMed
Search for other papers by A. A. F. Mahmoud in
Current site
Google Scholar
PubMed
A purified glycoprotein, glycoprotein-2, which was isolated from Schistosoma japonicum soluble egg antigen, was tested in an enzyme-linked immunoadsorbent assay for its potential as an immunodiagnostic reagent. Using empirically-derived criteria of endpoint titer, 39/40 sera from S. japonicum-infected individuals were scored correctly as positive in a blind test. Of these, 26/40 were from individuals who excreted fewer than 100 eggs per gram of stool. No cross reactions were observed with sera from uninfected individuals (0/20) or from individuals infected with S. mansoni (0/22), Trichinella spiralis (0/6) or Brugia malayi (0/4). Cross reactions were observed with 3/24 individuals infected with S. hematobium who excreted >2,000 eggs per 10 ml urine. Glycoprotein-2 appears to satisfy the requisite criteria of specificity and sensitivity to warrant further evaluation.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1110 | 620 | 25 |
| Full Text Views | 4 | 1 | 0 |
| PDF Downloads | 2 | 0 | 0 |