Preparation of a New Antivenin by Affinity Chromatography

F. E. Russell Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona

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J. B. Sullivan Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona

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N. B. Egen Biophysics Technology Laboratory, College of Engineering, University of Arizona, Tucson, Arizona 85721

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W. S. Jeter Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona

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F. S. Markland School of Medicine, University of Southern California, Los Angeles, California 90033

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W. A. Wingert School of Medicine, University of Southern California, Los Angeles, California 90033

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D. Bar-Or Emergency Department, Denver General Hospital, Denver, Colorado 80204

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DOI:
https://doi.org/10.4269/ajtmh.1985.34.141
Volume/Issue:
Volume 34: Issue 1
Page(s):
141–150
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Polyacrylamide gel affinity chromatography was employed to isolate and purify antibodies to the antigens of the venoms of four rattlesnakes. The antivenins were studied for their neutralizing properties on a number of pharmacologic preparations. It was found that the purified antibodies (IgG) were more efficacious than the commercially prepared antivenin in neutralizing the lethal, cytolytic, hemorrhagic, platelet aggregating, and other deleterious effects of the venoms. In addition, the purified antibodies gave no evidence of producing anaphylaxis or anaphylactoid reactions in animals sensitized to horse serum. The proposed technique is also more simple than current production methods, is time-saving, and less expensive.

Author Notes

Copyright:
Copyright © 1985 by The American Society of Tropical Medicine and Hygiene 1985
Accepted:
26 Jul 1984
|
Published Online:
Jan 1985
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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