Delayed Type Hypersensitivity Granuloma Formation Around Schistosoma Mansoni Eggs in Vitro

IV. Granuloma Formation in Human Schistosomiasis

Barbara L. DoughtyUniversity of Pennsylvania, School of Medicine, Department of Medicine, Allergy and Immunology Section, The Biomedical Research Center for Infectious Diseases, Philadelphia, Pennsylvania 19174, Egypt

Search for other papers by Barbara L. Doughty in
Current site
Google Scholar
PubMed
Close
,
Deni M. ZoddaUniversity of Pennsylvania, School of Medicine, Department of Medicine, Allergy and Immunology Section, The Biomedical Research Center for Infectious Diseases, Philadelphia, Pennsylvania 19174, Egypt

Search for other papers by Deni M. Zodda in
Current site
Google Scholar
PubMed
Close
,
Aziz El KholyUniversity of Pennsylvania, School of Medicine, Department of Medicine, Allergy and Immunology Section, The Biomedical Research Center for Infectious Diseases, Philadelphia, Pennsylvania 19174, Egypt

Search for other papers by Aziz El Kholy in
Current site
Google Scholar
PubMed
Close
, and
S. Michael PhillipsUniversity of Pennsylvania, School of Medicine, Department of Medicine, Allergy and Immunology Section, The Biomedical Research Center for Infectious Diseases, Philadelphia, Pennsylvania 19174, Egypt

Search for other papers by S. Michael Phillips in
Current site
Google Scholar
PubMed
Close
Restricted access

Peripheral blood mononuclear cells, obtained from 42 school-age Egyptian children, were isolated on Ficoll-hypaque density gradients and assayed for granuloma formation. A granuloma index (G.I.) which classified the cellular reactions to Schistosoma mansoni eggs was determined for each patient. Morphologic criteria to assess the cellular reactivity included cell adherence, blast cell transformation, cell migration, and circumoval accumulation of inflammatory cells around the egg. The difference between the mean granuloma index of uninfected controls (G.I. = 1.25 ± 0.04) and infected patients (G.I. = 1.58 ± 0.05) was statistically significant (P < 0.01; Student's t-test). There was no correlation between the granuloma indices and infection intensities determined by quantitative egg counts or between anti-major serological antigen antibody titers. These data demonstrate the feasibility of studying granulomatous hypersensitivity in human schistosomiasis utilizing an in vitro model of granuloma formation and peripheral blood cells.

Author Notes

Present address: Texas A&M University, College of Veterinary Medicine, Department of Veterinary Microbiology and Parasitology, College Station, Texas 77843.

Present address: Smith/Kline Diagnostics, Sunnyvale, California.

Save